Banca de QUALIFICAÇÃO: ERICSON RAMOS DE MELLO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : ERICSON RAMOS DE MELLO
DATE: 12/12/2022
TIME: 13:00
LOCAL: Sala 21 do Pavilhão Hugo de Souza Lopes (Anexo II) do ICBS/UFRRJ
TITLE:

MOLECULAR IDENTIFICATION OF COCCIDIANS OF WILD BIRDS: THE APPLICABILITY OF SOME GENIC REGIONS IN SPECIES DIFFERENTIATION AND PHYLOGENETIC ANALYSIS

KEY WORDS:

Isospora, Eimeria, oocysts, sequencing, molecular identification, Taxonomy, Phylogeny.

PAGES: 63
BIG AREA: Ciências Biológicas
AREA: Zoologia
SUBÁREA: Taxonomia dos Grupos Recentes
SUMMARY:

Coccidia are obligate intracellular protozoa that infect most invertebrates, all classes of vertebrates, including domestic and wild animals, and humans. Currently, there are several techniques and methodologies to be applied for the characterization of coccidian species, as host susceptibility, determination of parasite density, biological cycle patterns, pathogenicity, etc. which provide taxonomic adjustments relevant to species primarily identified by the morphology of the exogenous form, the oocyst. Studies in Molecular Biology in recent decades have stimulated the development of techniques for oocyst isolation, DNA extraction and amplification and sequencing of certain gene regions that have been relevant for the confirmation and delimitation of species, improving the taxonomy of coccidia, as well as promoting phylogenetic analyses. Based on these facts, this study aimed to identify, by molecular methods, coccidians from wild birds captured in Atlantic Forest regions of Southeastern Brazil, performing the following steps: (1) Isolating coccidian oocysts from fecal samples of wild birds, which have been previously identified morphologically; (2) Extract the DNA and amplify and sequence different genic regions of the COI and 18S genes; (3) Evaluate the applicability of these new sequences in species differentiation and phylogenetic studies. In this sense, this manuscript presents a review of the scientific literature on the theme of molecular identification of coccidians of wild birds, and in chapters I, II and III, the preliminary results of molecular identification of three coccidian species, which correspond to three articles published in international scientific journals. A final chapter (chapter IV), which is still under development, will appear in the final thesis manuscript and will deal with yet another new species of coccidia identified by morphological and molecular methods.

COMMITTEE MEMBERS:
Presidente - 1971595 - BRUNO PEREIRA BERTO
Externa ao Programa - 4073611 - AGUIDA APARECIDA DE OLIVEIRA - UFRRJExterna ao Programa - 3008869 - MICHELLE DANIELE DOS SANTOS CLAPP - UFRRJExterna ao Programa - 1764496 - VIVIANE MOREIRA DE LIMA - UFRRJ