Banca de DEFESA: ISADORA DOS SANTOS DIAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ISADORA DOS SANTOS DIAS
DATE: 01/03/2023
TIME: 09:00
LOCAL: Videoconferência
TITLE:

Molecular diagnosis of Rickettsia spp. in ticks parasitizing humans, domestic and wild animals circulating in areas belonging to Academia das Agulhas Negras, Resende, RJ, Brazil.

KEY WORDS:

Rickettsia sp., bacteria, sentries, Hydrochoerus hydrochaeris

PAGES: 59
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Numerous species of domestic and wild animals act as reservoirs of hemoparasites transmitted by ticks, causing hemoparasites of great importance to public health, such as Brazilian Spotted Fever. Known to cause high fever, this disease is a zoonosis with worldwide distribution and is caused by bacteria of the Rickettsiaceae family, presenting a complex diagnosis. The agent's detection has been facilitated due to access to molecular techniques, such as the polymerase chain reaction (PCR). Military personnel in training or operations, due to frequent activities and prolonged exposure, are more predisposed to infestations and at greater risk of infection by bacteria of the Rickettsiaceae family. Therefore, the objective of the present study was to carry out the molecular diagnosis of Rickettsia spp. in ticks parasitizing humans, domestic and wild animals circulating in areas belonging to Academia das Agulhas Negras, Resende, Rio de Janeiro, Brazil. A sampling of ticks infesting different species of vertebrate hosts (humans, wild and domestic animals) collected and identified by Prado (2022) during the years 2019 to 2021 were used. A stratified random sample of 494 ticks were selected to be molecularly tested for the presence of Rickettsia spp. corresponding to 52 tick samples on domestic animals, 138 tick samples on captured wild animals, 37 tick samples on small mammals, 200 tick samples on collared peccaries and 67 tick samples on military personnel. The samples were subjected to DNA extraction, followed by a prior amplification of the partial sequence of the nuclear ITS-2 gene of ticks (family Ixodidae) for quality control of the extraction and conventional PCR for the detection of target DNA corresponding to the gltA gene of ticks Rickettsia spp.. A sample of A. dubitatum was positive during molecular reactions, amplifying the gltA gene fragment of Rickettsia spp.. The corresponding sample belongs to Hydrochoerus hydrochaeris. This finding reveals that wild animals can harbor rickettsial agents, and raises the alarm about animals as sentinels and even humans who are in areas of vulnerability. It is necessary to deepen the study, in addition to carrying out the sequencing.

COMMITTEE MEMBERS:
Presidente - ***.018.557-** - ADIVALDO HENRIQUE DA FONSECA - UFRRJ
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Externa à Instituição - IZABELA MESQUITA ARAÚJO - UFRRJ
Externo à Instituição - RENATA FERNANDES FEREIRA DE MORAES - UV