Banca de DEFESA: THAMIRES REZENDE ARAÚJO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : THAMIRES REZENDE ARAÚJO
DATA : 24/07/2019
HORA: 09:00
LOCAL: sala 14
TÍTULO:

Presence of Trypanosoma vivax and Anaplasma marginale in Stomoxys calcitrans, fly-of-stables, on the campus of Universidade Federal do Rio de Janeiro, Seropédica - RJ

PALAVRAS-CHAVES:

Stomoxys calcitrans; Trypanosoma vivax; Anaplasma marginale;

PÁGINAS: 61
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Flies of the Stomoxys genus have been characterized as mechanical and biological vectors of several pathogens. Stomoxys calcitrans, a cosmopolitan fly with hematophagous males and females individuals, presents aggressive and persistent behaviour which leads it to feed in different hosts. Such factor contributes for the fly to be a mechanical vector of several pathogens. The objective of this study was to detect the presence of Trypanosoma vivax and Anaplasma marginale in Stomoxys calcitrans flies collected at three points inside the campus of the Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro. The flies collected were dissected, their organs were separated and stored for extraction of deoxyribonucleic acid (DNA) and molecular analysis by conventional PCR (cPCR) and semi nested PCR for Trypanosoma vivax and Anaplasma marginale. In cPCR assays, all the 55 samples were negative for the presence of T. vivax. In semi nested PCR assays, 19 samples were positive for the presence of A. marginale and the others were negative. This work is the first report of the presence of A. marginale in S. calcitrans at Rio de Janeiro state and also in our country, pointing that the epidemiological importance of this vector in the transmission of diseases with great economic impact must not be ignored. Although the flies collected at Seropédica campus of UFRRJ were negative for the presence of T. vivax, the collection of fly samples at other different regions of the state must considered, once the infection of cattle by this parasite have been recently reported at Rio de Janeiro state.

MEMBROS DA BANCA:
Externo ao Programa - 3103528 - CLAUDIA BEZERRA DA SILVA
Externo à Instituição - HELENA LÚCIA CARNEIRO SANTOS - FIOCRUZ
Externo à Instituição - LILIAN CRISTINA DE SOUSA OLIVEIRA BATISTA CIRNE - CESVA
Externo ao Programa - 3103478 - MARISTELA PECKLE PEIXOTO
Presidente - 1792175 - PATRICIA FAMPA NEGREIROS LIMA