Banca de QUALIFICAÇÃO: GABRIELA FRIANI VIEIRA TREVISANO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : GABRIELA FRIANI VIEIRA TREVISANO
DATA : 27/09/2019
HORA: 13:30
LOCAL: Sala 03
TÍTULO:

Use of Metarhizium anisopliae s.l. to control in Biomphalaria glabrata the intermediate host of Schistosoma mansoni

PALAVRAS-CHAVES:

Schistosomiasis; Biomphalaria glabrata; Entomopathogenic fungi; Biological control;

PÁGINAS: 55
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Schistosomiasis is a disease that affects 200 million people worldwide. There is currently no secure way to control its intermediate host. The present study aimed to evaluate the effects of Metarhizium anisopliae s.l. CG 347 against Biomphalaria glabrata snails, intermediate host of Schistosoma mansoni. Parasite-infected snails were exposed for 24 hours to the fungus and the following aspects of the snails were analyzed: snails’ survival, impact of M. anisopliae treatment in the cercarial shedding, number of circulating hemocytes in the hemolymph and and histophatlogy. The uninfected and fungus treated mollusks were analyzed concerning: survival, total protein concentration of hemolymph and digestive gland, scanning electron microscopy of shells and number of circulating hemocytes in the hemolymph. There was no mollusks’ death after the treatment period, but a reduction of 20% of the total protein content in the hemolymph was observed when compared to the water control. The shell of fungus-treated mollusks exhibited hyphae and blastospores. S. mansoni shedding of cercariae reduced 32.7% when snails were treated with M. anisopliae. The infected group had a lower number of hemocytes, and the group exposed to M. anisopliae and infected by S. mansoni showed an increase when compared to the previous group. Regarding the histopathology of the mollusks, the groups UNI + Ma and INF + Ma, colonization by M. anisopliae in the esophagus, stomach and intestine. This study addressed the first report of reduced S. mansoni cercarial shedding after treatment of snails using the entomopathogenic fungus M. anisopliae.

MEMBROS DA BANCA:
Interno - 2860277 - ISABELE DA COSTA ANGELO
Externo à Instituição - SIMONE QUINELATO BEZERRA - FIOCRUZ
Externo à Instituição - VINICIUS MENEZES TUNHOLI ALVES - UFRRJ