Banca de DEFESA: CAROLINA DE ALBUQUERQUE CORREIA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAROLINA DE ALBUQUERQUE CORREIA
DATA : 17/02/2020
HORA: 10:00
LOCAL: Sala de aula do Laboratório de Imunologia e Virologia- Instituto de Veterinária
TÍTULO:

Influence of B-1 lymphocytes on the leukocyte dynamics of Bruton tyrosine kinase deficient animals (XID)

PALAVRAS-CHAVES:

  B-1 lymphocytes, inflammation and modulation.

PÁGINAS: 60
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
ESPECIALIDADE: Saúde Animal (Programas Sanitários)
RESUMO:

The body's physiological response to an offending agent is inflammation and can be triggered by an infection and / or injury. The acute phase of this process is a rapid response and occurs within the first minutes and hours after pathogen recognition. Their resolution usually results in the elimination of infectious agents and repair of normal tissue architecture and function. The chronic phase begins when attempts to restore homeostasis are unsuccessful. Inflammation control through immune system components is a promising alternative to the use of conventional drugs that often do not meet patients' demands due to their multiple side effects. B-1 cells are capable of producing natural antibodies, may have T lymphocyte antigens and produce various cytokines, including the anti-inflammatory cytokine IL-10. Thus, they have the potential to modulate the inflammatory response. In this paper, we evaluated the population dynamics of leukocytes in the blood and peritoneal cavity of BALB / c and XID mice during the acute inflammatory response triggered by LPS. For this, we use XID mice, whose peritoneum is an environment devoid of B-1 cells. Our results revealed that XID animals spontaneously have a high number of peripheral blood neutrophils and this population becomes even larger after LPS inoculation. Concomitantly, high levels of IL-6 were detected. In addition, the peritoneal cavity of these animals also has a larger amount of neutrophils compared to BALB / c mice. This data does not change after stimulation in our experimental conditions. In the phagocytosis assays, we observed that the number of macrophages capable of phagocytosis is statistically equal between BALB / c and XID, but the number of internalized yeasts is lower in the LID and IFN-γ stimulated XID group. This suggests greater microbicidal power of the macrophages of these mice. This result is corroborated by the nitrite dosage in the culture supernatant, in which the stimulated XID macrophages produced more nitric oxide than the control group. The body of facts points to the development of a more intense inflammatory response in XID mice compared to BALB / c mice, probably due to impaired production of anti-inflammatory cytokine IL-10. Thus, our analysis reports the unprecedented finding of increased neutrophil population in the blood and peritoneal cavity of XID mice, confirms the importance of B-1 lymphocytes in modulating the inflammatory response, and suggests that these may be future targets for investigations into immunotherapy strategies.

MEMBROS DA BANCA:
Presidente - 1222756 - DEBORA DECOTE RICARDO DE LIMA
Interna - 1545840 - LUCIA HELENA PINTO DA SILVA
Externa ao Programa - 1335855 - DANIELLE DE OLIVEIRA NASCIMENTO
Externo à Instituição - LEONARDO MARQUES DA FONSECA - UFRJ
Externo à Instituição - LEONARDO FREIRE DE LIMA - UFRJ