Hemoparasites of ticks from forest fragments in the Eastern Amazon and anthropized areas of the Atlantic Forest, Brazil.
Carrapatos; Bioagentes Transmitidos por Carrapatos; Amazônia; Mata Atlântica;
Although the Amazon Forest and the Atlantic Forest are among the most important biomes in Brazil, they are regions that suffer the consequences of human actions. This contributes to the emergence of diseases caused by pathogens, due to human contact with the area of circulation of pathogens and their vectors. This study aimed to detect hemoparasites in anthropized areas of the Amazon Forest and regions of the Atlantic Forest. Ticks were collected in a forest fragment in Pará and in the Iguaçu National Park in Paraná, as well as ticks and fleas in wild animals in the state of Rio de Janeiro. In order to obtain these ectoparasites, techniques such as dragging, flannel leggings and CO2 traps were used, as well as for inspection on wild animals. Ectoparasites were identified using taxonomic keys or molecular identification. DNA extraction was performed to assess the presence of Rickettsia spp., Ehrlichia spp., Anaplasma spp., Borrelia spp. and Piroplasmida through PCR. A total of 1,508 ticks were obtained, identified in 17 species: Amblyomma spp., A. cajennense s.s., A. calcaratum, A. geayi, A. goeldii, A. humerale, A. naponense, A. pacae, A. varium, A. sculptum, A. dubitatum, A. longirostre, A. brasiliense, A. ovale, A. incisum, A. parkeri, H. juxtakochi and A. coelebs. A total of 54 fleas were obtained, all identified as C. f. felis. Rickettsia bellii was detected in A. varium, A. goeldii and A. dubitatum; Rickettsia spp. in A. longirostre, A. ovale and A. pacae; Rickettsia felis and Rickettsia sp. showing high similarity with “Candidatus R. senegalensis” in C. f. felis; Anaplasma spp. em A. brasiliense e A. coelebs; Wolbachia sp. em C. f. felis; Borrelia sp. with high similarity to Borrelia turcica in A. calcaratum, A. coelebs and A. ovale; e Piroplasmida in A. sculptum, A. longirostre and A. coelebs. All samples tested were negative for Ehrlichia spp. This work brings the first record of R. bellii in A. varium and in A. goeldi; the first record of Rickettsia sp. with high similarity to “C. R. senegalensis ”in Brazil; and increases the number of tick species of the genus Amblyomma infected with Borrelia sp. close to B. turcica.