Banca de DEFESA: GERALDO AUGUSTO PEREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GERALDO AUGUSTO PEREIRA
DATE: 01/03/2021
TIME: 14:00
LOCAL: Plataforma Google meeting
TITLE:

Development of polymeric film containing aqueous extract of Psidium guajava L. for the treatment of periodontitis in dogs

KEY WORDS:

 Periodontal disease, guava, antimicrobials, veterinary dosage forms

PAGES: 81
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Periodontal disease is the most common oral disease affecting both men and animals, predominantly occurring in dogs over three years old. It’s result of the progressive inflammatory response due to the accumulation of bacterial plaque and the signs and symptoms include halitosis, pain, accumulation of dental calculus, enlarged of the gingival pouch, severe inflammation and infections, destruction of the gums, teeth and bones, bacteremia with progression to septicemia. Plants extracts like the one from Psidium guajava L have antimicrobial activity and the potential for use in the prevention and treatment of oral diseases. Popularly known as guava tree [goiabeira] in Brazil, the Psidium guajava L. is used worldwide in traditional medicine and in the food industry. The objective was to characterize the antimicrobial and antioxidant activity of the aqueous extract of the guava leaf and the development and quality control of a modified oral mucoadhesive release formulation containing extract, for the treatment and prophylaxis of periodontal disease in dogs. Microbiological methods such as disk diffusion and broth microdilution, chemical methods such as thin layer chromatography, antioxidant analysis (DPPH), total phenolics were used. In addition, uniformity of weight, pH, swelling index, disintegration time, infrared and sterility of the films. The aqueous extract obtained yield of 1.2%, found in thin layer chromatography the presence of flavonoids, antioxidant capacity of EC50 = 140 µg.mL^-1 and total phenolics of 17.02 ± 6.87 mg equivalent of gallic acid per g of dry extract. In the evaluation of microbial susceptibility, maximum inhibition halos (mm) were obtained for resistant S. aureus as 18 ± 3.46, for sensitive S. aureus 16 ± 0, for resistant S. pseudintermedius 17.3 ± 1.15, for sensitive S. pseudintermedius 18 ± 0 and for sensitive beta-hemolytic Streptococcus 21 ± 2.00, with a concentration of 3.6 mg / cm² at medium. In the broth microdilution test, the minimum inhibitory concentration (MIC) for all strains of 8.8 mg.mL-1 and the minimum bactericidal concentration (CBM), only for S. pseudintermedius (resistant) was 8.8 mg. mL-1, for the other strains it was greater than 11.4 mg.mL-1. The buccal film information presents homogeneity and malleability, having a pH compatible with the buccal one (from 6.35 ± 0.07 to 6.45 ± 0.18), degree of swelling from 27.0 ± 62.8% to 369.8 ± 28.0%, and the disintegration time in approximately 4 minutes. The physical infrared test evidenced that there were no interactions between the components and the base itself. The results demonstrated that it was possible to develop a polymeric film of oral dissolution with release in melting having antioxidant and antimicrobial capacity.

BANKING MEMBERS:
Presidente - 1700427 - YARA PELUSO CID
Interno - 1705075 - DOUGLAS SIQUEIRA DE ALMEIDA CHAVES
Interna - 1722406 - SHANA DE MATTOS DE OLIVEIRA COELHO
Externa à Instituição - MARIA BERNADETE RIEMMA PIERRE - UFRJ
Externa à Instituição - NEIDE MARA DE MENEZES EPIFANIO