Banca de DEFESA: LEANDRA OLIVEIRA MOREIRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LEANDRA OLIVEIRA MOREIRA
DATE: 31/03/2022
TIME: 09:00
LOCAL: Videoconferência
TITLE:

In vitro activity of Ocimum gratissimum L. Essential Oil on Ctenocephalides felis felis (Bouché, 1835) and Rhipicephalus sanguineus (Latreille, 1806).

KEY WORDS:

Key words: Ocimum gratissimum L. Ectoparasites. Insecticide.

PAGES: 75
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SUMMARY:

         The flea Ctenocephalides felis felis and the tick Rhipicephalus sanguineus are the main ectoparasites that affect dogs and cats, as they are species present all over the world, and with vector capacity of a series of diseases for both animals and humans. The control of infections by ectoparasites of veterinary importance still depends a lot on the use of chemical substances that reach the nervous system of arthropods. Such compounds have suffered from a number of drawbacks, such as the development of resistance and concerns for animal, human and environmental safety. Therefore, the search for safer and more effective products and technologies has been a challenge for the industry, it is in this context that the studies and development of products from natural origins gain relevance. In the area of veterinary medicine, we have studies in the control of ticks and fleas with the essential oil of Ocimum sp that exhibit a range of biological activities such as insecticide, acaricide and insect repellency. Therefore, this work aimed to evaluate the potential of the essential oil of Ocimum gratissimum L. in the control of immature and adult forms of the flea Ctenocephalides felis felis and the tick Rhipicephalus sanguineus. Among the specific objectives are the obtaining and chemical characterization of the essential oil from the leaves of Ocimum gratissimum L., the evaluation of the in vitro activity in different phases of the cycle of the flea Ctenocephalides felis felis (egg, larva, pupa and adults) and the tick Rhipicephalus sanguineus (larva, nymph and adults), and the determination through probit analysis of the LC50 and LC90 of the essential oil of the leaves of Ocimum gratissimum L. in different phases of the cycle of the flea Ctenocephalides felis felis and the tick Rhipicephalus sanguineus. We observed that the essential oil of Ocimum gratissimum L. presented 100% mortality in all analyzed flea stages. For the tick we can observe that in the larval stage we reached 100% mortality, while the maximum mortality reached for nymphs was 80.8%, and in the adult stage we did not reach mortality. The analysis of the chemical composition of the essential oil of O. gratissimum showed that the main compounds found were Eugenol (77.7%) and Pinene (5.26%). We determined by probit analysis the following LC50 and LC90: egg (LC50 = 10.54; LC90 = 22.00), larva 24h (LC50 = 21.06; LC90 = 59.79), larva 48h (LC50 = 13.20; LC90 = 38.56), pupa (LC50 = 18.69; LC90 = 69.26), adult 24h (LC50 = 20.24; LC90 = 55.90), adult 48h (LC50 = 15.72; LC90 = 44.72) and ID (CL50 = 2.00; CL90 = 5.18). It was possible to obtain, characterize, evaluate the insecticidal and acaricidal potential and determine through probitus analysis the LC50 and LC90 of the essential oil of Ocimum gratissimum L. in all phases of the flea cycle Ctenocephalides felis felis (egg, larva, pupa and adults) ) and the tick Rhipicephalus sanguineus (larva, nymph and adults).        

 

 

 

 

BANKING MEMBERS:
Presidente - 1700427 - YARA PELUSO CID
Interno - 124.088.737-03 - DIEFREY RIBEIRO CAMPOS - UFRRJ
Interna - 2929889 - THAIS RIBEIRO CORREIA AZEVEDO
Externo à Instituição - ISABELLA VILHENA FREIRE MARTINS - UFES
Externa à Instituição - NEIDE MARA DE MENEZES EPIFANIO