Banca de DEFESA: CRISTIANE DA SILVA ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : CRISTIANE DA SILVA ALVES
DATE: 21/08/2024
TIME: 09:00
LOCAL: Sala 34-ICBS
TITLE:

Evaluation of the aqueous extract of Eleusine indica in the life cycle of Caenorhabditis elegans: a model organism used to screen products with anthelmintic activity.

KEY WORDS:

Caenorhabditis elegans, Eleusine indica, nematode, phenolics, plant extract

PAGES: 64
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Helminths represent a major public health problem for pets and farm animals, leading to economic losses in the agricultural sector and the risk of zoonoses to their owners. Plant extracts and their components can be an efficient alternative to anthelmintics. The objective of this study was to chemically and biologically characterize the aqueous extract of Eleusine indica and evaluate its antioxidant capacity and anthelmintic action against the nematode Caenorhabditis elegans. To achieve this objective, the extract was characterized in its phenolic components by antioxidant and quantitative tests, and its activity against the adult nematode was performed using concentrations of 50 to 1500 μg/mL-1 in quintuplicate and incubated for 48h. A 10% (w/v) aqueous extract of E. Indica was obtained. After analysis, the product presented 5.50 mg of quercetin/g of dry extract of flavonoids and 8.15 mg of quercetin/g of dry extract for phenolics. When the values of total antioxidants were investigated, the plant extract obtained the value of 63.71 mmol Fe(II)/100 mg of dry extract. Its nematicidal activity indicated LC50 and LC90 of 725.6 and 12926.0 μg/mL-1, respectively. Therefore, the extract of Eleusine indica presented a nematicidal effect in the proposed animal model, and its antioxidant capacity was evaluated.

COMMITTEE MEMBERS:
Presidente - 1705075 - DOUGLAS SIQUEIRA DE ALMEIDA CHAVES
Interna - 1722406 - SHANA DE MATTOS DE OLIVEIRA COELHO
Externo ao Programa - 1809123 - ANDRE MARQUES DOS SANTOS - UFRRJExterna à Instituição - NEIDE MARA DE MENEZES EPIFANIO
Externa à Instituição - VIVIANE DE SOUZA MAGALHAES