Banca de DEFESA: MARCELA BARLETTE MENDES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARCELA BARLETTE MENDES
DATE: 23/08/2024
TIME: 15:00
LOCAL: Sala 74 IV
TITLE:

METHICILLIN-RESISTANT STAPHYLOCOCCUS IN A DAIRY FARMING ENVIRONMENT – A ONE HEALTH APPROACH

 

KEY WORDS:

Dairy cattle farming, Antimicrobial Resistance, One Health.

PAGES: 53
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Infecciosas de Animais
SUMMARY:

Dairy cattle farming represents one of the main sources of income and job creation in the Brazilian agricultural sector, being marked by marked heterogeneity both in relation to production systems and the profile of herds and producers. The health status of a dairy herd is essential within the production chain, where the biggest difficulty faced by producers is mastitis. Its etiology is attributed to both environmental and contagious agents, with bacteria of the genus Staphylococcus being the most common cause among all cases and widely distributed throughout the world. The animal production sector becomes crucial in the propagation of both pathogens and resistance genes, with the administration of antimicrobials as growth promoters and prophylactics being the main route of selective pressure on the animal and environmental microbiota. Considering the significant risk posed by infectious conditions and the economic embargoes involved, the WHO declared antimicrobial resistance a substantial threat to global health. The objective of this work was to evaluate the prevalence of species of the genus Staphylococcus and the phenogenotypic profile of antimicrobial resistance against the class of beta-lactams mediated by the mecA and blaZ genes from isolates obtained from samples of bovine milk collected on properties in the south of the state of Rondônia and Baixada Fluminense, as well as the hygienic-sanitary and socioeconomic profile of the producers. The sampling comprised 127 milk samples, which were subjected to phenotypic identification at the genus level in AMVF, tube coagulase test and resistance to bacitracin 0.04UI, and proteomic identification at the species level using the MALDI-TOF technique. The characterization of the phenotypic resistance profile was carried out using the disk diffusion technique given by cefoxitin, oxacillin and penicillin, and the detection of the mecA and blaZ genes through PCR. The survey of the hygienic-sanitary and socioeconomic profile of the properties was carried out by applying a questionnaire during sample collection. The results obtained demonstrated a prevalence of approximately 58% of S. aureus, compared to approximately 42% of CNS species. Phenotypic antimicrobial resistance was observed in only 35% of penicillin-resistant isolates, of which approximately 43% came from the North region and 57% from the Southeast region. Among these, the presence of the mecA gene was confirmed in around 20% of the isolates, with a prevalence of 15% and 25% for the North and Southeast regions, respectively; and blaZ in 7% of the isolates, which were obtained only from samples from the Southeast region. The results obtained provided a comparison of the resistance profile of the isolates and the hygienic-sanitary and socioeconomic profile and properties included in the study. In this way, the need for approaches aimed at meeting the particularities of each region is evident, making deeper studies on the topic essential.

COMMITTEE MEMBERS:
Presidente - 2212438 - MILIANE MOREIRA SOARES DE SOUZA
Externo à Instituição - BRUNO GOMES DE CASTRO - UFMT
Externo à Instituição - FELIPE CARLOS DUBENCZUK