Banca de DEFESA: THAMYRIS SAMPAIO VILELA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : THAMYRIS SAMPAIO VILELA
DATA : 17/06/2019
HORA: 09:00
LOCAL: Sala 24
TÍTULO:

Natural infection by parasites of the genus Plasmodium spp. in Culex sp. captured in peridomiciliary areas in the Municipality of Seropédica, state of Rio de Janeiro

PALAVRAS-CHAVES:

Food Soure; Avian malaria; Real-time PCR

PÁGINAS: 86
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Avian malaria is an arbovirose that has a wide geographical distribution, being in Brazil the Plasmodium juxtanucleare species that causes this pathology in birds of the species Gallus gallus. The affected birds present several symptoms ranging from weight loss to neurological signs, causing considerable loss in the poultry industry when the flock is affected. Although the parasite vertebrate host interaction has an emphasis in the studies, little is known about the vector parasite interaction in the dynamics of this disease. Some experimental studies have demonstrated the ability of Plasmodium juxtanucleare to be vectored by some Culex species. The objective of this study was to identify Culex species infected by Plasmodium naturally, determining the vectoring potential of some species, as well as to identify the food source of these dipterans by the real-time multiplex PCR technique, allowing a better elucidation on the parasite- vector. The species of Culicinae found in this study were Culex quinquenfasciatus, C. nigripalpus, Aedes aegypiti, A. albopictus, A. albifasciatus, Anopheles aquasalis, with Culex being the predominant species at the study site. The occurrence of diploid infection by Plasmodium spp. showed a correlation with the dietary habits of these insects in birds, especially in May, November and December, which were hot, humid and rainy months, increasing Culex sp. in the region and emphasizing the influence of climatic conditions on the occurrence of the vector and consequently its vectoring capacity. This study also allowed the elaboration of two high sensitivity multiplex PCR techniques, and each technique was able to detect up to three food sources at the same time. Through the analyzes of food preference of Diptera genus Culex, it was possible to correlate the positivity of infection to Plasmodium spp. with their eating habits. In this way, a greater preference for sources of repast in birds was observed, even with the presence of other available animal species, evidencing an ornitofilic character of mosquitoes of the genus Culex

MEMBROS DA BANCA:
Externo à Instituição - ALINE MOREIRA DE SOUZA - UFF
Externo à Instituição - DANIEL DA SILVA GUEDES JUNIOR - FIOCRUZ
Presidente - 3701492 - HUARRISSON AZEVEDO SANTOS
Externo à Instituição - MARCUS SANDES PIRES - UFF
Externo ao Programa - 3103478 - MARISTELA PECKLE PEIXOTO
Externo ao Programa - 2688704 - TIAGO MARQUES DOS SANTOS