Evaluation of entomopathogenic nematodes infection and reinfection on Stomoxys calcitrans (Linnaeus, 1958) (Diptera: Muscidae) at varying exposure times.
Stable fly, entomopathogenic nematodes, biological control.
Stomoxys calcitrans (Linnaeus, 1758), it’s a haematophagous dipterous popularly known as the "stable fly", It is able to parasite several animal species, its distribution is worldwide, with population increase in the hottest periods of the year. The parasitism of this fly is a problem to livestock, leading to considerable losses around the world. Due to the resistance of pests to chemical pesticides, added to the worldwide demand for food free of traces of these substances, different microorganisms have been researched in order to verify their applications in the control of parasites of economic importance. In this context, several agents appear as alternatives for use in biological control, among them are fungi, bacteria, viruses and entomopathogenic nematodes (EPN). EPN have high virulence for arthropods; they are mobile, easily grown and have great reproductive potential. This work was divided into two chapters. Chapter I aimed to evaluate the effect of H. bacteriophora (HP88) and H. baujardi (LPP7) infection on S. calcitrans larvae at different exposure times; Chapter II aimed to evaluate the effect of EPN H. bacteriophora (HP88) and H. baujardi (LPP7) reared on S. calcitrans larvae on third instar larvae of the stable fly, and to verify the emergence rate of adult flies. In Chapter I, it was observed that EPN H. bacteriophora presented mortality rates of 51.7, 83.3 and 91.7%, for the 12, 24 and 48 hours, respectively, while for H. baujardi were 9.3 (12h), 35 (24h) and 35% (48h). When evaluating exposure times for each nematode, it is observed that for H. bacteriophora, the two longest exposure times (24 and 48 h) were effectively more efficient for the others, although they were statistically equal between them. For H. baujardi, a slight variation was observed between the times. In Chapter II the mortality rates were 15% for the control group, 35% for H. bacteriophora and 25% for H. baujardi, the mortality rate of H. bacteriophora was statistically higher than the control group, but statistically the same as H. baujardi. When assessing the adult emergence rate, it was observed that there was no statistical difference between the treated and control groups, where the overall average was 70.54%. It was concluded that the EPN are promising in the control of third instar larvae of S. calcitrans. H. bacteriophora-HP88 is the most suitable for use in stable fly control (Chapter I), and that H. bacteriophora-HP88 and H. baujardi- LPP7 when isolated from S. calcitrans larvae are not efficient in the control of stable third instar larvae of the stable fly, nor do they cause negative effects on the emergence of adult flies (chapter II).