Banca de DEFESA: ANA CAROLINE FERREIRA DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ANA CAROLINE FERREIRA DE SOUZA
DATA : 05/03/2020
HORA: 09:00
LOCAL: Anexo 1 IV - LQEPV
TÍTULO:

Standardization of the technique and in vitro infection of Ctenocephalides felis felis larvae by entomopathogenic nematodes Heterorhabiditis bacteriophora (strain HP88) and Heterorhabiditis indica (strain LPP30).

PALAVRAS-CHAVES:

Ctenocephalides felis felis, entomopathogenic nematodes, biological control.

PÁGINAS: 66
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Fleas of the species Ctenocefalides felis felis, also known as cat fleas, are hematophagous ectoparasites of the order Siphonaptera. Several animal species can be parasitized, including man. The fleas are widely distributed and are of great importance in Veterinary Medicine, which can cause severe itching and allergic dermatitis in pets, in addition to being vectors of various pathogens such as rickettsiae, bacteria, worm cestodes, nematodes and protozoa, both in animals and in humans. The control is done through integrated management of the environment and animals infested through chemical products. Current studies demonstrate that the inappropriate use of such chemicals to control this insect has become an item of great concern due to the occurrence of resistant populations in some regions. Thus, the search for natural products that act in biological control, such as fungi, bacteria, viruses, protozoa and entomopathogenic nematodes has become important in insect control. In recent years, the study on entomopathogenic nematodes (EPNs) has increased, making this an alternative in the control of insects of veterinary importance, as they present several advantages in relation to their use, and can therefore be used alone or in conjunction with Chemicals. The present study aimed to standardize and evaluate the susceptibility of seven-day-old flea larvae of the species Ctenocephalides felis felis by entomopathogenic nematodes of the genus Heterorhabiditis. The study was divided into two stages, where the first three bioassays aimed to standardize the infection technique of flea larvae of C. felis felis, verifying the amount of liquid necessary for the nematodes to move and infect the larvae (400, 600 and 1000 µl); to evaluate the susceptibility of infection and mortality of flea larvae by entomopathogenic nematodes Heterohabiditis bacteriophora (strain HP88) in three different concentrations (120, 160 and 200 EPNs/flea larvae). The second bioassay aimed to evaluate the susceptibility to infection and mortality of C. felis felis larvae by the entomopathogenic nematode Heterohabiditis indica (strain LPP30) in three different concentrations (120, 160 and 200 EPNs/flea larvae). All bioassays were divided into two groups, one containing a specific diet for larvae and the other without a diet. Parametric data were evaluated by analysis of variance (ANOVA) followed by the Tukey test (p <0.05). In the first three bioassays with H. bacteriophora (HP88), it was possible to standardize the infection of larvae of C. felis felis, in Petri dishes with 6 cm in diameter, where the most adequate concentration of nematodes containing liquid was 600 µl, showing significant difference when compared to the bioassay that used 400 µl. In the bioassay in which the 1000 µl volume was used, mortality was 100%, including in the control groups, suggesting that the amount of liquid is not suitable for infection. Still for the species H. bacteriophora (HP88), in the volume of 600µl, there was no significant difference between the groups with and without diet, nor in the concentration of EPNs used for the infection. In the second stage of the study, infection of the larvae of C. felis felis by entomopathogenic nematodes H. indica (LPP30) was proven, both in the group with diet and in the group without diet, as well as in the three concentrations of entomopathogenic nematodes, with no significant difference between them. Through these results, it can be suggested that in vitro infection of seven-day flea larvae of the species Ctenocephalides felis felis by the species Heterorhabditis bacteriophora (HP88) and Heterorhabditis indica (LPP30) is possible, which is a tool to be studied for the use in the biological control of these insects.

MEMBROS DA BANCA:
Presidente - 2221840 - MELISSA CARVALHO MACHADO DO COUTO CHAMBARELLI
Interno - 2181861 - AVELINO JOSE BITTENCOURT
Interna - 2221855 - PATRICIA SILVA GOLO
Externa à Instituição - CLÁUDIA DE MELO DOLINSKI - OUTRO
Externa à Instituição - SIMONE QUINELATO BEZERRA - FIOCRUZ