Banca de DEFESA: TATIANA WERNECK FRANCO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : TATIANA WERNECK FRANCO
DATA : 19/02/2020
HORA: 09:00
LOCAL: Sala 14 Departamento de Parasitologia Animal
TÍTULO:

Analyisis of the culturable microbiota asscoiated with a laboratory colonly of Ctenocephalides felis felis (Siphonaptera, Pulicidae)

 

PALAVRAS-CHAVES:

ectoparasites, microbial communities,  PCR,  antibiotic resistance

PÁGINAS: 70
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

The flea (Ctenocephalides felis) is the principal ectoparasite of dogs and cats worldwide acting as a vector of zoonotic pathogens including Rickettsia felis and Bartonella. The diversity of host associated microbes and their interactions within their hosts, including arthropods, are fundamental to the ecological functions within those communities and may contribute to host evolution. Moreover, symbiotic interactions may influence the transmissions of zoonotic pathogens, via effects upon vectoral competence. Knowledge of the microbiota of vectors has been used to develop novel approaches for control based on the concept of microbiota manipulation. A key component in this strategy is the presence of a stable/core microbiota. The present study characterized the stability, over seven years, of the cultivable microbiota of a laboratory colony of C. felis. Bacteria associated with different life stages were isolated by culture on plates of nutrient agar. Bacteria were identified, to the genus or species level, by polymerase chain reaction (PCR) amplification of a 500 base pair (bp) fragment of the gene encoding prokaryotic 16S ribosomal RNA, followed by nucleotide sequencing. Cultures were characterized for susceptibility to seven antimicrobial compounds (ampicillin, chloramphenicol, mercury chloride, nitrofurantoin, oxytetracycline, rifampicin and streptomycin), using a microdilution method. Each of the different life stages presented a unique microbiota, however a core component of all samples were members of the genus Staphylococcus, with some demonstrating multiple-drug resistant phenotypes. The most prevalent species were S. saprophyticus, S. nepalensis, S. lentus and S. cohnii all of which,  have been reported as opportunistic pathogens with zoonotic potential. The constant presence of the same species of Staphylococcus, in multiple life stages, suggests they are essential components of the microbiota and by implication of the biology of the fleas. Future research will examine the effects of manipulating the core microbiome as the first step in the development of novel strategies for infestation control. 

 

MEMBROS DA BANCA:
Presidente - 1354903 - DOUGLAS MCINTOSH
Interna - 2929889 - THAIS RIBEIRO CORREIA AZEVEDO
Externa ao Programa - 1815763 - IRENE DA SILVA COELHO
Externa à Instituição - MARIA HALINA OGRZEWALSKA - FIOCRUZ
Externo à Instituição - STEFAN SCHWAB - EMBRAPA