Banca de QUALIFICAÇÃO: PATRICIA GONZAGA PAULINO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : PATRICIA GONZAGA PAULINO
DATE: 23/02/2022
TIME: 09:00
LOCAL: Plataforma Google Meet
TITLE:

Characterization of Rhipicephalus (Boophilus) microplus Sialotranscriptome in response to Theileria equi infection

KEY WORDS:

equine piroplasmosis; RNA-seq; tick-protozoan interactions.

PAGES: 102
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

This study intends to characterize the sialotranscriptome profile of Rhipicephalus (Boophilus) microplus in response to Theileria equi and identify genes of interest with differential genomic expression, indicating relevant targets in the tick–protozoan interactions. The experimental design consisted of RNA sequencing from uninfected and T. equi-infected R. microplus salivary glands (SGs) to obtain transcriptomic profiles for characterization and comparison. A total of 288,952 transcripts were obtained from both tick profiles, 3456 transcripts (p < 0.05) differentially expressed in response to T. equi infection. The uninfected SGs’ registered 231,179 transcripts, of which 155,359 were annotated. The most transcribed sequences were female-specific histamine binding protein and lipocalins. Regarding the T. equi-infected SGs, from the 238,964 assembled transcripts, 163,564 were annotated. The most transcribed sequences were histone demethylase JARID1 and Y-box-binding protein. Five transcripts (cystatin, arginase, nuclear factor κB kinase inhibitor subunit β (IκB), IκB delta, lysosomal-trafficking regulator, and reeler protein) presented the gene ontology (GO) category “response to protozoan” and were exclusively displayed in the T. equi-infected profile. The transcriptome of T. equi was also analyzed, registering 4728 hits. The study’s genetic and molecular information would be of great value for future studies and biotechnological applications envisaging disease control. The achievements of this project can be applied to future tick proteins studies in veterinary medicine. The genetic and molecular information obtained in this study would be of great value for biotechnological applications; it could be employed in a variety of T. equi studies or even be extrapolated to other similar haemoparasites in veterinary medicine.

BANKING MEMBERS:
Presidente - 3701492 - HUARRISSON AZEVEDO SANTOS
Interna - 2221855 - PATRICIA SILVA GOLO
Externa ao Programa - 3247559 - BRUNA DE AZEVEDO BAETA
Externo à Instituição - DANIEL DA SILVA GUEDES JUNIOR - FIOCRUZ
Externa à Instituição - NATHALIE COSTA DA CUNHA - UFF