Pulicidal efficacy and in vitro residue of the essential oil of Illicium verum against Ctenocephalides felis felis (BOUCHÉ, 1835) (SIPHONAPTERA: PULICIDAE).
Essential oil. Fleas. Control. Ectoparasites.
The Ctenocephalides felis felis flea is the main ectoparasite of dogs and cats, transported worldwide. Around 50% of the dermatological pathologies observed in animals are related to the presence of C. felis felis. Due to its biology, large infestations are frequent, and the use of chemical insecticides to control infestations is very common. However, these products have disadvantages related to environmental degradation and resistance by target organisms. This panorama has supported research in search of compounds with insecticidal activity with potential for the development of products that may represent alternatives to those currently used, increasing studies with botanical insecticides. Illicium verum is a medicinal plant of the Schisandracea family, with fruits rich in essential oil and with bioactivity against several insects. The present study aimed to determine the chemical composition of the essential oil of Illicium verum fruits, evaluate its in vitro pulicidal activity on adult Ctenocephalides felis felis fleas as well as its residual efficacy. Commercial essential oil was used. Chemical analysis by gas chromatography identified 16 compounds, with (E)-anethole being the major constituent representing 79.96% of the composition. Use the paper filter strip impregnation method to perform the in vitro test. The essential oil of Illicium verum showed insecticidal activities against adults of Ctenocephalides felis felis, with 100% efficacy at a concentration of 800μg • cm-2. To carry out the residual efficacy test, the filter paper method was used with the concentration that presents 100% mortality, the mortality of the fleas was evaluated every 24 hours, after each evaluation how fleas were replaced by new fleas from the colony, kept as impregnated bulletin tapes, this procedure was repeated every 24 hours until no further purifying efficacy was verified. Bioactivity was observed over 18 days.