Banca de DEFESA: GABRIELA PEREIRA SALCA DE ALMEIDA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : GABRIELA PEREIRA SALCA DE ALMEIDA
DATE: 25/02/2022
TIME: 09:00
LOCAL: https://us02web.zoom.us/j/89464091596?pwd=VXgzNWxvSTVQbXh0T1dLRWVTS0d1UT09
TITLE:

Development of cryopreservation techinques of Caenorhabiditis elegans (Nematoda, Rhabditida) for laboratory culture maintenance

KEY WORDS:

nematode; cryoprotectant agent; glycerol; polyvinylpyrrolidone

PAGES: 53
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The nematode Caenorhabditis elegans has been used as an experimental model for studies in several sectors of biology and medicine due to the low economic costs for maintenance, cultivation and execution of experiments. The use of cryopreservation technique for this species can generate a source of genetically stable living tissues and cells for a variety of purposes. Currently, standard preservation techniques for C. elegans have shown difficulties to obtain results with high success rates in terms of survival. Thus, it is necessary to constantly develop new techniques and tests for new substances that can guarantee greater viability and stability in the conservation of cultures. The present study sought to define a cryopreservation methodology using different substances and associations in order to improve the maintenance and transport of the C. elegans culture. Three determining factors for survival and culture maintenance capacity of C. elegans during freezing and thawing were considered, namely the use of a preservative agent, the association with a gelling agent and the storage time in liquid nitrogen, using freezing in two steps. The use of glycerol and polyvinylpyrrolidone (PVP) showed satisfactory results in the association of gelling agents such as agar, carbopol and carboxymethylcellulose (CMC), respectively. Polyethylene glycol (PEG) and polysorbate 80 (T80) compared to the other substances tested, did not obtain significant efficiency, despite confirming their cryoprotective character between repetitions. There were no results demonstrating that these components evaluated individually brought benefits to the cryopreservation of C. elegans. Considering the unfolding of the factors among themselves for the survival of C. elegans, it was identified that the maintenance of individuals alive after thawing from seven days of storage is essentially dependent on the use of preservatives associated with some type of gelling agent.

BANKING MEMBERS:
Presidente - 2929889 - THAIS RIBEIRO CORREIA AZEVEDO
Interno - 387340 - CARLOS WILSON GOMES LOPES
Externa ao Programa - 057.185.477-00 - VIVIANE DE SOUZA MAGALHAES - UFRRJ
Externo à Instituição - FERNANDO DE ALMEIDA BORGES - UFMS
Externa à Instituição - TASSIA TORRES FURTADO - UFRRJ
Externo à Instituição - WENDELL MARCELO DE SOUZA PERINOTTO - UFRB