Banca de DEFESA: TAIS MEDEIROS SIQUEIRA CARVALHO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : TAIS MEDEIROS SIQUEIRA CARVALHO
DATE: 18/07/2022
TIME: 14:00
LOCAL: VIDEO CONFERÊNCIA
TITLE:

Molecular investigation of trypanosomatids in equine and bovine blood samples

KEY WORDS:

Trypanosomatids; Brazilian herds; Trypanosoma theileri

PAGES: 65
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Trypanosomatids of Veterinary Medical Importance are unicellular, flagellated protozoa transmitted to a wide variety of vertebrate hosts. These pathogens determine significant economic losses, as well as some of them cause zoonotic diseases, impacting human health, especially in developing countries. The occurrence of these diseases has been reported in animals in the Americas, since there is an abundant presence of hematophagous insects, enabling the dissemination and adaptation of these parasitic agents. In the present study, molecular assays were performed to detect agents of the Trypanosomatidae family, in blood samples from horses from different locations in Brazil and from cattle from the southern region of Rio de Janeiro. In the equine samples, molecular diagnosis was used, through the conventional polymerase chain reaction (cPCR) to detect agents of the Trypanosomatidae family. Whole blood samples were used, where no positives were detected in 225 samples from animals from Rio de Janeiro, 89 samples from the state of Mato Grosso do Sul, 343 samples from the state of Pará and 109 samples from the state of Acre. In the municipality of Vassouras, RJ, blood samples were collected from 226 cattle in 8 dairy farms for hematocrit determination, preparation of blood smears for direct parasitological examination and molecular diagnosis. At Nested-PCR examination, 2 animals of the same property were positive for the Trypanosoma genus. The genetic sequencing of these samples revealed that they were the species Trypanosoma theileri. Molecular detection studies of these agents can reveal the circulation of these pathogenic agents in animal herds of national production and alert about the forms of transmission, as well as elucidate cases of productive losses on properties.

BANKING MEMBERS:
Presidente - ***.018.557-** - ADIVALDO HENRIQUE DA FONSECA - UFRRJ
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Interna - 3103478 - MARISTELA PECKLE PEIXOTO
Externo à Instituição - ADLILTON PACHECO DE OLIVEIRA - UES
Externa à Instituição - ANA PAULA MARTINEZ DE ABREU - UV