Banca de DEFESA: RAFAELA SOUZA ABDO ELIAS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RAFAELA SOUZA ABDO ELIAS
DATE: 16/10/2023
TIME: 09:00
LOCAL: Sala 3 do Departamento de Parasitologia Animal
TITLE:

Epidemiologic analysis of hemoparasites in dogs in rural areas of the Vale do Paraíba region, in the State of Rio de Janeiro

KEY WORDS:

dog, epidemiology, rural area, hemoparasite

PAGES: 58
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

The objective of this research was to carry out an epidemiological analysis through the detection of Babesia vogeli and Ehrlichia canis using the real-time PCR (qPCR) technique in blood samples from dogs domiciled in seven municipalities in the Vale do Paraíba region, located in the Rio de Janeiro state. The cross-sectional observational study was carried out to verify the association of environmental and host factors with the presence of B. vogeli and E. canis DNA in the blood of dogs from these regions, through statistical tests such as bivariate analysis (Fisher's exact test). and multivariate analysis with the construction of predictive models for infections by B. vogeli and E. canis. A total of 384 blood samples were collected from dogs from the municipalities of Seropédica, Paracambi, Piraí, Barra do Piraí, Barra Mansa, Pinheiral and Vassouras, located in the sul of Rio de Janeiro. From the blood collection, the genetic material was extracted and subjected to detection of specific fragments of the Heat shock protein 70 kDa (hsp 70) and ribosomal subunit (16S rRNA) genes for B. vogeli and E. canis, respectively, through application of the qPCR technique. An epidemiological questionnaire was developed and applied to dog owners, addressing environmental factors and characteristics related to the host that could be associated with the occurrence of infection by B. vogeli and E. canis. Among the thirteen animals in which B. vogeli was detected, 5% (n = 2/40) belonged to Barra do Piraí, 5.27% (2/38) belonged to Barra Mansa, 10% (n=1/10) belonged to Paracambi, 3.77% (n=2/53) belonged to Piraí, 12% (n=3/25) belonged to Vassouras, 12.5% (n=1/8) belonged to Pinheiral and 16.6 % (n=2/12) belonged to Seropédica. While of the 20 animals in which E. canis was detected, 5% (n=2/40) belonged to Barra do Piraí, 7.90% (n=3/38) belonged to Barra Mansa, 10% (n=1/ 10) belonged to Paracambi, 15.09% (n=8/53) belonged to Piraí, 8% (n=2/25) belonged to Vassouras, 25% (n=2/8) belonged to Pinheiral and 16.6 % (2/12) belonged to Seropédica. The result of the epidemiological analysis of this study was that there was no association between the variables and the presence of Babesia vogeli and Ehrlichia canis. Meanwhile, logistic regression demonstrated that dogs that live in the same environment are three times more likely to become infected with E. canis (OR = 3.52; CI 1.21 – 10.24; p = 0.02). This study found that the presence of more than one dog per property is a relevant factor and is associated with E. canis infection in the target area of this work.

COMMITTEE MEMBERS:
Presidente - 3701492 - HUARRISSON AZEVEDO SANTOS
Interno - 386163 - CARLOS LUIZ MASSARD
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Externa à Instituição - ALINE MOREIRA DE SOUZA - UFF
Externa à Instituição - ANA PAULA MARTINEZ DE ABREU - UV