Banca de DEFESA: LAÍS FELICIANO DE SOUZA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LAÍS FELICIANO DE SOUZA
DATE: 22/11/2023
TIME: 09:00
LOCAL: Sala 14 do Departamento de Parasitologia Animal
TITLE:

Anaplasma marginale in Rhipicephalus microplus, Stomoxys calcitrans and Haematobia irritans: Molecular detection and epidemiological analysis in ticks

KEY WORDS:

Rhipicephalus microplus, Stomoxys calcitrans, Haematobia irritans, molecular diagnosis, Anaplasma marginale.

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Bovine anaplasmosis is a disease caused by the intracellular bacterium Anaplasma marginale (Rickettsiales: Anaplasmataceae) which is widely distributed and can cause major economic damage to countries where cattle farming is carried out. Cattle of all ages can be affected by this disease, and there may be asymptomatic or symptomatic cases, leading to reduced milk production, abortions and even death. Rhipicephalus (Boophilus) microplus is one of the vectors of A. marginale in Brazil, but other forms of transmission have also been reported, including mechanical transmission by hematophagous flies and iatrogenic transmission. Among the factors that contribute to the difficulty of controlling bovine anaplasmosis, we can highlight the doubts regarding the possible vectors, means and mechanisms of transmission. The aim of this study was to investigate the presence of Anaplasma marginale DNA in Rhipicephalus (Boophilus) microplus ticks and in Stomoxys calcitrans and Haematobia irritans flies on dairy farms in the municipalities of Seropédica and Paracambi in the state of Rio de Janeiro. In the laboratory, the specimens of each arthropod were dissected, and the internal organs used to extract deoxyribonucleic acid (DNA), evaluated by real-time PCR (qPCR) and nested PCR for molecular analysis of A. marginale and sequenced to validate the results. As a result, 73% (n=29/40) of S. calcitrans, 90% (n=26/29) of H. irritans and 45% (n=43/96) of R. microplus were positive. This study reports the presence of A. marginale DNA in the salivary glands of R. microplus females as well as in the mouthparts of S. calcitrans and H. irritans flies. The results presented in this study reveal a high frequency of A. marginale DNA in the arthropod species investigated, highlighting the importance of new experimental research studies to prove the participation of these arthropods in the transmission cycle. In this way, it will be possible to implement strategic measures for the prevention of bovine anaplasmosis, taking into account the species of arthropod vectors that participate in the epidemiological chain of the disease in cattle in Brazil and worldwide.

COMMITTEE MEMBERS:
Presidente - 3701492 - HUARRISSON AZEVEDO SANTOS
Interna - 3103528 - CLAUDIA BEZERRA DA SILVA
Interna - 3103478 - MARISTELA PECKLE PEIXOTO
Externo à Instituição - DANIEL DA SILVA GUEDES JUNIOR - FIOCRUZ
Externa à Instituição - PRISCILLA NUNES DOS SANTOS - UV