Banca de DEFESA: MONIQUE TAVEIRA MEDEIROS

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : MONIQUE TAVEIRA MEDEIROS
DATE: 19/12/2024
TIME: 14:00
LOCAL: Sala Prof. Laerte Grisi e https://meet.google.com/vas-osqd-fyy
TITLE:

Bioprospecting of Volatile Natural Compounds Against Cochliomyia hominivorax Larvae

KEY WORDS:

essential oil, green chemistry, screwworm, control

PAGES: 67
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

The objective of this work was to evaluate the in vitro insecticidal potential of 15 different EOs and the main constituents of anethole, carvacrol and thymol against Cochliomyia hominivorax larvae. In this study, L3 of the C. hominivorax fly maintained in a laboratory colony were used. The insecticidal activity of EOs of: Citrus bergamia, C. paradisi, Cinnamomum cassia, Copaifera reticulata, Cymbopogon flexuosus, C. winterianus, Eugenia caryophyllus, Illicium verum, Juniperus virginiana, Lavandula hybrida, Origanum vulgare, Pelargonium graveolans, Rosmarinus offcinalis, Salvia sclarea and Thymus vulgaris and the major constituents: anethole, carvacrol and thymol and the associative effect of the binary combination of the three major constituents. The evaluation of biological activity was carried out by contacting L3 with filter paper impregnated with different concentrations of EO. The first step was to determine the insecticidal activity of the 15 EOs by exposing the larvae to a concentration range that varied from 2000 – 200 μg/cm². For the EOs that presented a mortality rate of 100%, lethal concentrations (LC) 50 and 90 were determined, as well as their majority constituents. The data were tabulated, and the average mortality percentage was calculated for 24 and 48 hours after exposure. Next, LC50 and LC90 were calculated using the RStudio Team® program and the associative effect was calculated using the CompuSyn version 1.0 program, with a statistical significance level of p < 0.05 and confidence interval (95% CI). In the first stage, the mortality percentage was equal to 100% for the EOs of I. verum, O. vulgare and T. vulgaris. The EOs of C. bergamia, C. paradisi, C. cassia, C. flexuosus, E. caryophyllus and R. officinalis presented a mortality rate ranging from 30 to 60%. The others had a mortality rate of less than 30%. The LC₅₀ was 417.9 and 559.4 μg/cm² and the LC₉₀ was 913.9 and 1028.1 μg/cm² for I. verum EO and anethole, respectively, in the evaluations after 48 hours. For the OE of O. vulgare, the LC₅₀ were 540.9 and 253.71 μg/cm² and the LC₉₀ were 1819.3 and 1193.9 1 μg/cm² for evaluations after 24 and 48 hours. For carvacrol, the LC₅₀ was 970.5 and 931.9 μg/cm² and the LC₉₀ was 1700.2 and 1591.8 μg/cm² for evaluations after 24 and 48 hours. For the EO of T. vulgaris, the LC₅₀ were 407.1 and 314.8 μg/cm² and the LC₉0 were 1149.4 and 589.7 μg/cm² for evaluations after 24 and 48 hours. For thymol, LC₅₀ were 255.7 and 102.3 μg/cm² and LC₉₀ were 1008.6 and 690.1 μg/cm² for evaluations after 24 and 48 hours. In the analysis of the associative effect of binary combinations of the majority constituents, it was possible to prove an antagonistic effect of anethole with carvacrol and thymol and a synergistic effect of carvacrol with thymol. Based on the results presented, it is possible to conclude that EOs can be an alternative for the control of C. hominivorax larvae, especially the EOs of I. verum, O. vulgare and T. vulgaris, as well as their majority constituents.

 

COMMITTEE MEMBERS:
Presidente - ***.088.737-** - DIEFREY RIBEIRO CAMPOS - UFRRJ
Interna - 2929889 - THAIS RIBEIRO CORREIA AZEVEDO
Interna - 1700427 - YARA PELUSO CID
Interna - ***.630.647-** - BARBARA RAUTA DE AVELAR - UFRRJ
Externo à Instituição - ISABELLA VILHENA FREIRE MARTINS - UFES
Externo à Instituição - GUILHERME MARCONDES KLAFKE
Externo à Instituição - LIVIO MARTINS COSTA JUNIOR