Banca de DEFESA: RAISSA COUTO SANTANA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : RAISSA COUTO SANTANA
DATA : 22/02/2019
HORA: 09:00
LOCAL: Anfiteatro do DPA-Sala 14
TÍTULO:

AVALIAÇÃO DO POTENCIAL MICROBICIDA DOS COMPOSTOS 1.8 CINEOL, ΑLFA-PINENO E P-CIMENO PRESENTES NO ÓLEO ESSENCIAL DO GÊNERO ProtiumSOBRE Leishmaniaamazonensisin vitro

PALAVRAS-CHAVES:

Leishmania amazonensis, terpenes, Protium, treatment

PÁGINAS: 93
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Leishmaniasis is one of the wide neglected diseases in the world. According to WHO, the number of cases can reach 1.3 million annually. The clinical manifestations of these diseases afflict humans and animals, being a public and veterinary health problem. The treatment used has several problems such as difficult administration, toxicity, high cost and cases of resistance have been reported. Essential oils are products derived from plants composed of different substances that can present various therapeutic actions, such as the terpenes as 1.8 cineol, P-cymene and α-pinene. In this work we study the potential microbicide of this compounds present in essential oils of Protium genus on Leishmania amazonensis in vitro, one of the etiological agents of cutaneous and diffuse cutaneous leishmaniasis in the New World. Our data show that murine macrophages infected with L. amazonensis promastigotes and treated with the compounds 1,8-cineol, α-pinene and P-cymene have an anti-amastigote activity. The compounds inhibited the amastigotes  survival in a dose-dependent manner with IC₅₀ of 38.8 μg/ml, 48 μg/ml and 52.75 μg/ml for α-pinene, P-cymene and 1,8-cineol, respectively. The cytotoxicity for murine macrophages was evaluated by XTT and the results showed that the cells remained viable at 70-95% up to the highest tested concentration (200μg / ml), as well as their phagocytic capacity was not altered by treatment. Nitric oxide production by macrophages was analyzed because it is a highly toxic molecule for intracellular microorganisms, however the treatment of  resting or LPS-activated macrophages did not modulate NO production by macrophages. Another possible mechanism of action analyzed was the effect of treatment on parasite lipid composition, our results showed that treated -L. amazonensis promastigotes had a change in the lipid profile. It was observd alteration on triacylglycerol, sterol + 1,2-diacylglycerol, fatty acids, 1,3-diacylglycerol and stratified sterols in 48h treatment at a concentration of 50 μg/ml. The results indicate that the compounds α-pinene, P-cymene and 1,8-cineol are potential drug candidates for the treatment of leishmaniasis.

MEMBROS DA BANCA:
Externo ao Programa - 1735975 - CEDRIC STEPHAN GRAEBIN
Externo à Instituição - DEIVID COSTA SOARES - UFRJ
Externo à Instituição - JULIANY COLA FERNANDES RODRIGUES - UFRJ
Presidente - 1545840 - LUCIA HELENA PINTO DA SILVA
Interno - 1792175 - PATRICIA FAMPA NEGREIROS LIMA