Banca de DEFESA: RAPHAEL FRANCISCO DUTRA BARBOSA DA ROCHA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : RAPHAEL FRANCISCO DUTRA BARBOSA DA ROCHA
DATA : 08/08/2019
HORA: 10:00
LOCAL: Laboratório de Viroses-IV
TÍTULO:

Immunomodulatory effect of B-1 cells and B-1 derived phagocytes (B-1CDP) in experimental Chaga’s Disease by Trypanosoma cruzi

PALAVRAS-CHAVES:

B-1 cells; B-1CDP cells; Trypanosoma cruzi; susceptibility

PÁGINAS: 83
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

The hemoflagellate protozoan Trypanosoma cruzi causes Chagas disease; although it has been described over a century ago this disease is still a matter of public health. B-1 cells and/or B-1 phagocyte derived cells (B-1CDP) contribute to the survival of several pathogens. Recent works highlight that over infection by T. cruzi, the B-1 cells execute an immunomodulatory function over phagocytic cells making then more susceptible to the parasite. With this information in mind we decided to evaluate the influence of B-1 and B-1CDP cells during clone Dm28c metacyclic trypomastigotes T. cruzi infection. In parallel, to some cultures were added in the proportion of 10:1 B1 cells to macrophages in vitro. Our results showed that macrophages from BALB/c mice are more susceptible to the T. cruzi infection then macrophage from XID mice. We also demonstrate an increased number of infected cells; with higher numbers of amastigotes forms inside macrophages and more extrusion of trypomastigotes forms. B-1 cells were responsible by the reduction of nitric oxide (NO), influencing the microbicide capacity of this macrophages. The presence of B-1 cells was determinant for the higher susceptibility of BALB/c mice cells and for the brake of tolerance of XID macrophage. In parallel, the B-1 cells induced an increased production of IL-10 cytokine, this one able to modulate the macrophage response to infection by T. cruzi. We also evaluated the possible in vivo effects; and we designed an experiment where BALB/c and XID mice received by adoptive transfer B-1 cells before the intra-peritoneal infection with T.cruzi. Additionally, BALB/c mice that received B-1 cells shown a higher susceptibility when compared with mice that do not received this cells during the course of infection with T.cruzi. Taken together, our work suggests that B-1 and B-1CDP cells contribute leading to decreased resistance favoring parasitism in T. cruzi infection.

MEMBROS DA BANCA:
Externo ao Programa - 1335855 - DANIELLE DE OLIVEIRA NASCIMENTO
Presidente - 1222756 - DEBORA DECOTE RICARDO DE LIMA
Externo à Instituição - HELOISA D`AVILLA DA SILVA BIZARRO - UFJF
Externo à Instituição - HERBERT LEONEL DE MATOS GUEDES - FIOCRUZ
Externo à Instituição - LEONARDO FREIRE DE LIMA - UFRJ
Interno - 1545840 - LUCIA HELENA PINTO DA SILVA
Interno - 1792175 - PATRICIA FAMPA NEGREIROS LIMA