Banca de DEFESA: CECILIA LOPES DA CONCEICAO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CECILIA LOPES DA CONCEICAO
DATA : 21/02/2020
HORA: 09:00
LOCAL: Sala Prof Laerte Grisi - LQEPV
TÍTULO:

In vitro insecticidal activity of three essential oils on Ctenocephalides felis felis

PALAVRAS-CHAVES:

Fleas; Biocontrol; Cinnamomum cassia; Thymus vulgaris; Origanum vulgares;

PÁGINAS: 84
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Fleas are insects of great importance in veterinary medicine. There is an increasing number of researches and tutors of dogs and cats that seek the use of products of natural origin that, in theory, are less toxic to animals, and that cause less damage to the environment. The objective of this work was to determine the chemical composition of the essential oils of Cinnamomum cassia; Thymus vulgaris; Origanum vulgare, assess insecticidal activity, calculate lethal concentrations 50 and persistence of effectiveness. The chemical composition was made by Gas Chromatography (GC) equipped with a flame ionization detector (FID), and a split / split-less injector to separate and detect the constituents of the essencential oils (OEs). To carry out the in vitro test, a stock solution of EOs was prepared and diluted in 20% acetone, in different concentrations. To assess insecticidal activity, ten adult fleas, ten eggs, ten larvae and ten pupae were used. Ten eggs were used to assess inhibition of development. And in the effectiveness persistence test ten adult fleas were used. The adult phase was placed in test tubes (1x10 cm), and then a filter paper tape impregnated with the different concentrations of each EO was inserted. For the tests in the immature phase, a petri dish (60x15mm) was used, and a disc of filter paper impregnated with the same concentrations was inserted. The number of live and dead insects was recorded after a certain exposure time. The mortality percentage was determined using the following formula: mortality (%) = number of dead insects X 100 / number of incubated insects. For statistical evaluation, the data found were tabulated and the Probit analysis was performed using the IBM SPSS Statistics computer program to calculate the CL50 and CL90 values with a 95% confidence interval. The major constituents were: cinnamaldehyde for C. cassia OE, thymol for T. vulgaris and carvacrol for O. vulgaris. Regarding the insecticidal activity, it was possible to obtain the LC50 of the EOCC was 64.3; 3.0; 10.3; 34.6 and 2.3μg / cm2; for EOOV it was 21.8; 94.8; 57.7; 94.8; 6.2μg/cm2; and for EOTV it was 44.7; 13.5; 19.8; 203.4; 4.7μg/cm2 for the adult, egg, pupae larvae and development inhibition stages respectively. In the persistence of effectiveness, it was possible to observe that EOOV showed greater persistence of effectiveness, when compared to the others. It is concluded that each tested EO presents a better insecticidal activity for each phase of C. felis felis.

MEMBROS DA BANCA:
Interna - 122.630.647-02 - BARBARA RAUTA DE AVELAR - UFRRJ
Interno - 124.088.737-03 - DIEFREY RIBEIRO CAMPOS - UFRRJ
Externa à Instituição - GREICIANE FRANÇA BRONZATO DE ALMEIDA - UV
Presidente - 2456316 - KATHERINA COUMENDOUROS