Banca de DEFESA: GRAZIELE CALIXTO SOUZA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : GRAZIELE CALIXTO SOUZA
DATA : 13/03/2020
HORA: 09:00
LOCAL: IV - Anexo I - CPGCV - sala 14
TÍTULO:
Efficacy evaluation of entomopathogenic nematodes exposed to different temperatures of vinhoto in the control of larvae of Stomoxys calcitrans (Linnaeus, 1758) (Diptera: Muscidae).
PALAVRAS-CHAVES:
Sugar cane; stable fly; infective juveniles
PÁGINAS: 37
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
ESPECIALIDADE: Doenças Parasitárias de Animais
RESUMO:
The use of mechanized harvesting and the processing of sugar cane nowadays, generate by-products that help in the proliferation of Stomoxys calcitrans (Linnaeus, 1758), which affects various species of domestic animals and man, causing economic losses due to the occurrence of outbreaks . With the constant use of chemicals to control the fly in the stables, resistance to them is observed and this has led to further studies in biological control, where entomopathogenic nematodes (NEP) appear as organisms with potential use for biological control . The objective of the present study was to verify the efficiency of Heterorhabditis bacteriophora - HP88 and H. baujardi - LPP7, maintained at different temperatures of vinasse on S. calcitrans larvae in the laboratory. Each experimental unit consisted of ten third instar larvae placed in plastic pots containing four milliliters of the 50% vinasse solution. In each treatment, 300 infectious juveniles of H. bacteriophora - HP88 line or H. baujardi - LPP7 line were added per larva in the vinasse solution described above, heated for ten minutes at temperatures of 25, 28, 31, 34, 37 and 40 ° C, the treated groups and the control groups of the experiment were kept in an air-conditioned BOD chamber at 25 ± 1 ° C and 70 ± 10% RH. In total, 6 repetitions were performed for each treatment, with mortality observed daily for a period of 15 days. The results were evaluated, and in the statistical analysis, using the GLIMMIX procedure at 5% probability, it was found that there was no statistical difference between the treated groups, control groups and temperatures for larval mortality rate (P = 0, 8573), percentage of dead pupae (P = 0.1782) and adult emergence (P = 0.4386). When only the groups with presence and absence of NEPs were evaluated, larval mortality rates of 30% and 14.17% were observed for H. bacteriophora – HP88 line and H. baujardi - LPP7 line respectively, while the control groups presented 3,89% in H. bacteriophora – HP88 strain and 8.61% in H. baujardi - LPP7 strain. In the evaluation of temperatures, significant statistical differences were observed only in the temperatures 37 and 40 ºC of H. baujardi - strain LPP7 in the larval mortality rate. In pupal mortality rates, the group with H. bacteriophora - HP88 strain showed averages of 32.5, 34.17 and 27.5% at temperatures of 25, 31 and 34 ºC, respectively. In the group with H. baujardi - lineage LPP7, temperatures of 37 and 40 ºC presented 40% and 37.5% of pupal mortality. In the analysis of the adult emergence rate, there was a lesser emergence at temperatures of 25, 28, 31 and 34 ºC in the group with the presence of H. bacteriophora – strain HP88 and the group with H. baujardi - strain LPP7 with the lowest rates of emergence of adults at temperatures of 37 and 40 ºC. The results of the study show that there was a decrease in the mortality of the immature stages of S. calcitrans caused by NEPs maintained at different temperatures of vinasse, when compared to other studies without heating it. There is a need for further studies to adapt the use of NEP together with vinasse, heated or not.
MEMBROS DA BANCA:
Presidente - 2181861 - AVELINO JOSE BITTENCOURT
Interna - 2860277 - ISABELE DA COSTA ANGELO
Interna - 2221840 - MELISSA CARVALHO MACHADO DO COUTO CHAMBARELLI
Externa à Instituição - GISELA LARA DA COSTA - FIOCRUZ