Banca de DEFESA: MIGUEL ANGELO LEITE MOTA JÚNIOR

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MIGUEL ANGELO LEITE MOTA JÚNIOR
DATE: 17/08/2020
TIME: 09:00
LOCAL: A defesa erá realizada utilizando a plataforma Google Meet
TITLE:

Detection and molecular characterization of the Chicken Infectious Anemia virus in subsistence and industrial broilers in the State of Rio de Janeiro.

KEY WORDS:

Immunosuppression, Chicken Infectious Anemia, phylogenetic analysis, Industrial Breeding, Subsistence Breeding, Gallus gallus.

 

PAGES: 58
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Patologia Animal
SPECIALTY: Patologia Aviária
SUMMARY:

Chicken Infectious Anemia is an acute and contagious disease that leads to immunosuppression in young birds. Being a disease of great impact in the poultry sector, affecting the growth rate, providing a great non-uniformity of the flock, besides the capacity of association of the etiologic agent with other immunosuppressive diseases. The Chicken Infectious Anemia virus (CIAV) has a genetic material of ADN nature, consisting of 3 protein molecules that are crucial components for understanding the behavior of the virus. The objective of this study was to detect the molecular characterization of the CIAV VP1 gene from samples of broilers in industrial and subsistence farms in the state of Rio de Janeiro, Brazil. CIAV was detected using conventional PCR (“Polymerase Chain Reaction”), in different organs, such as: thymus, spleen, liver, cecal tonsil and bursa of Fabricius. From 60 sampled birds (30 from industrial farm and 30 from subsistence) it was possible to detect the presence of CIAV in 21.6% (n = 13/60). In livestock farms, a frequency of 20% (n = 6/30) was observed, while in industrial farms the frequency was 23.33% (n = 7/30) (p> 0.05). The virus was most frequently detected in the thymus and spleen (21.6%, n = 13/60), both in industrial and livestock farms. It is worth mentioning that in none of the evaluated farms the birds were vaccinated against CIAV. Subsequently, two positive samples from the livelihood system and two from the industrial system were sequenced and compared with other sequences available from GenBank to study the genetic diversity of viral strains. Phylogenetic analysis showed that samples 12 and 13 - Subsistence Creation and samples 31 and 36 - Industrial Creation were grouped into a single clade, which reveals that the strains are phylogenetically related. The results presented in this study allow us to conclude that CIAV circulates in farms of industrial creation and subsistence in the state of Rio de Janeiro. However, it cannot be said that livestock livestock are sources of infection for industrial farms. Monitoring using molecular tools for the detection of CIAV in industrial farms is extremely important to assess whether prevention methods are being effective in preventing the introduction of the virus.

BANKING MEMBERS:
Presidente - 3701492 - HUARRISSON AZEVEDO SANTOS
Externo ao Programa - 386067 - PAULO CESAR AUGUSTO DE SOUZA
Externo ao Programa - 2688704 - TIAGO MARQUES DOS SANTOS
Externa à Instituição - ALINE MOREIRA DE SOUZA - UFF
Externo à Instituição - DANIEL DA SILVA GUEDES JUNIOR - FIOCRUZ