Banca de DEFESA: AMANDA COUTO SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : AMANDA COUTO SILVA
DATE: 20/09/2021
TIME: 09:00
LOCAL: Plataforma Google Meet
TITLE:

Role of B-1 lymphocytes in the immunopathogenesis of systemic lupus erythematosus

KEY WORDS:

B-1 lymphocytes; systemic lupus erythematosus; immunomodulation; autoimmunity

PAGES: 88
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Saúde Animal (Programas Sanitários)
SUMMARY:

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that affects dogs, cats, horses, humans and primates. The pathogenesis is multifactorial, being associated with genetic, hormonal and environmental factors. SLE leads to deposition of immune complexes in joints and various organs, resulting in clinical manifestations. The participation of T and B lymphocytes is crucial for the pathogenesis of the disease. B-1 lymphocytes, a subpopulation of B cells, have characteristics that may contribute to the pathogenesis of autoimmune diseases because they are capable of secreting cytokines such as IL-10, exerting a modulating action on both the acute and chronic inflammatory response, they present antigens on T cells, they participate in innate and adaptive immunity and are the main producers of natural antibodies. Understanding the role of B-1 cells in the immunopathogenesis of SLE may open up fronts for studies on immunotherapeutic strategies that enable the control of this complex and multifactorial disease. The present work evaluated the role of B-1 cells in the immunopathogenesis of SLE using the pristane induction model. SLE was induced in BALB/c, XID (B-1 deficient) and XID female mice repopulated with B-1. The animals were evaluated for six months. We found that BALB/c pristane and XID repopulated with B-1 showed characteristic signs of the disease, such as lipogranuloma formation and splenomegaly. Notably, BALB/c pristane also presented ascites, joint edema, arthritis, kidney damage with immune complex deposition. In the spleen, animals with B-1 had a higher percentage of B cells (CD19+, IgM+), BALB/c pristane than CD8 T (CD3+, CD8+), while XID pristane had increased TCD4 (CD3+, CD4+). In the peritoneal lavage, after induction with pristane, there was a decrease in B cells (CD19+, IgM+) and an increase in B1a (CD19+ IgM+ CD5+) in BALB/c pristane. XID pristane showed an increase in TCD4 (CD3+, CD4+) and BALB/c pristane than TCD8 (CD3+, CD8+). In peripheral blood, BALB/c had a higher number of lymphocytes, XID had a neutrophilic profile, and in all SLE groups there was an increase in monocytes. The cytokines IL-10, IL-6 and IFN-gama were increased in repopulated BALB/c and XID. Based on these results, we suggest that the presence of B-1 cells may contribute to the development of SLE.

BANKING MEMBERS:
Presidente - 1222756 - DEBORA DECOTE RICARDO DE LIMA
Interna - 1545840 - LUCIA HELENA PINTO DA SILVA
Externa ao Programa - 1335855 - DANIELLE DE OLIVEIRA NASCIMENTO
Externa à Instituição - HELOISA D`AVILLA DA SILVA BIZARRO - UFJF
Externo à Instituição - HERBERT LEONEL DE MATOS GUEDES - FIOCRUZ