Banca de QUALIFICAÇÃO: SILVIO RODRIGUES DA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : SILVIO RODRIGUES DA SILVA
DATE: 01/10/2021
TIME: 14:00
LOCAL: Google Meet
TITLE:

Epidemiological and laboratory study of the main endemic hemoparasitosis in dogs treated at veterinary clinics in Rio de Janeiro, Brazil

KEY WORDS:

Ehrlichia spp., Anaplasma spp., Babesia spp., epidemiology, diagnostic methods, Accuracy

PAGES: 177
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

The small animal veterinary clinic plays an important role in the diagnosis, treatment, control and prophylaxis of the main diseases that affect dogs. In this context, hemoparasitosis represent the most common parasitic disease, regardless of gender, age and race, being considered endemic in the urban environment. Despite efforts to combat this disease, many animals still suffer from the disease, as many of the methods to control its vectors have not been effective. Due to this reality, the first chapter aimed to analyze the laboratory and epidemiological aspects involved in the prevalence of blood parasites in the urban environment. From a laboratory point of view, animals with thrombocytopenia, activated and anemic monocytes were the ones that presented the highest risk of being infected by hemoparasites. From an epidemiological point of view, the most relevant aspects were the size of the animal and the presence of ticks. The second chapter presented as objective to analyze the seroprevalence of the main endemic hemoparasites in the small animal clinic in 5 veterinary clinics using a commercial kit ELISA test. Of the 1132 selected exams, the seroprevalence of hemoparasites was 44.3% for at least one agent, being Ehrlichia spp. (33.3%), the most prevalent gender, followed by Dirofilaira immitis (7.1%), Anaplasma spp. (3.7%) and B. burgdorferi (0.3%). Regarding co-infection cases, the seroprevalence in the study population was 16.2% for at least two bioagents analyzed, with the highest seroprevalence of Ehrlichia spp. + Anaplasma spp. (7.9%), followed by Ehrlichia spp. + D. immitis (6.4%). The third chapter aimed to evaluate the accuracy of three diagnostic techniques for the agents, Ehrlichia spp. Anaplasma spp. and Babesia spp. in the routine of the small animal clinic. Samples from 70 domiciled dogs were collected for convenience. Comparisons were made between positive and negative animals in serological and molecular tests regarding the main signs and symptoms. All animals included presented at least 3 clinical signs and symptoms and laboratory alterations suggestive of hemoparasitosis. Blood samples were analyzed by smear, serology and qPCR. Sensitivity, specificity, positive and negative predictive values were calculated. The prevalence of Ehrlichia spp was 40% and 37.1% Anaplasma spp. was 34.3% and 14.3% and Babesia spp. was 8.6% and 14.3% in the ELISA and qPCR tests, respectively. The general parasitism of the 3 agents for the 3 techniques ranged from 1.43% to 82.86%. The ELISA test showed the highest sensitivity for the diagnosis of Ehrlichia spp. (Se= 37.93%) and the lowest sensitivity for the diagnosis of Anaplasma spp. (Se= 8.33%). The dot-ELISA test showed the sensitivity (Se=33.33%) and the highest PPV (93.33%) for the diagnosis of Babesia spp. Animals with epistaxis were 100% positive for Ehrlichia spp. in ELISA and 80% in PCR. Real time PCR was considered the fundamental technique for the diagnosis of Ehrlichia spp. Anaplasma spp. and Babesia spp. being possible to determine to select animals with subclinical, acute and chronic disease.

BANKING MEMBERS:
Presidente - 1148670 - TERESA CRISTINA BERGAMO DO BOMFIM
Interno - 2545823 - WAGNER DE SOUZA TASSINARI
Externo à Instituição - LUCIANO ANTUNES BARROS - UFF
Externa à Instituição - HELENA LÚCIA CARNEIRO SANTOS - FIOCRUZ
Externa à Instituição - NATHALIE COSTA DA CUNHA - UFF