Banca de DEFESA: FERNANDO ROCHA MIRANDA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : FERNANDO ROCHA MIRANDA
DATE: 07/03/2022
TIME: 13:00
LOCAL: Videoconferência
TITLE:
In vitro Evaluation of Synergistic Activity Between Fipronil, Eugenol and Carvacrol against Larvae, Nymphs and Non-Fed Adults of Rhipicephalus sanguineus sensu lato (Latreille, 1806)
KEY WORDS:
Ticks, In vitro test, Natural Control
PAGES: 67
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:
Dogs are no longer companion animals to become members of the family where the concern for the health and well-being of these individuals increases and linked to this, pharmacology is also driven in the search for increasingly effective medicines and insurance to control infestations. Rhipicephalus sanguineus, or brown dog tick, are arthropods that have high public health relevance due to their high capacity for disease transmission, as well as their irritating and despoiling potential, whether in the larval, nymphal or adult. Currently, its control is done with synthetic acaricides such as fipronil for the most part, but natural products have gained more and more space not only for their effectiveness, but also for their safety, such as the monoterpene carvacrol and the phenylpropanoid eugenol. The present work presents two steps where the first is the adaptation and standardization of a bioassay technique to test acaricide compounds against the non-fed adult stage of R. sanguineus, which consisted primarily in the adaptation and comparison of three known techniques for testing against the larval stage which are Larval Immersion Test (LIT), Monteiro's methodology and Larval Packet Test (LPT) which proved superior when compared to the others, presenting a lower fipronil LC50 value, which was 1236 µg/mL, against 5598.8 µg/mL for LIT and 4834.8 µg/mL for Monteiro, being the technique chosen to obtain the LC50 of fipronil, eugenol and carvacrol used for the synergistic evaluation against all unfed phases. Carvacrol and eugenol showed excellent pharmacological synergism for all stages, including adults, a stage that presents great resistance when compared to immature stages. The synergism coefficient values did not exceed 0.548, proving the potential of this interaction. On the other hand, fipronil showed incompatibility with eugenol and carvacrol, showing antagonism and decreased mortality in all concentration ratios used for both molecules. Studies with the adult stage of R. sanguineus are few, this work was the first to develop a test methodology with unfed adult ticks and to evaluate the synergism between natural and synthetic molecules, opening doors for future experiments in order to insert each time more botanical components to combat ectoparasites.
BANKING MEMBERS:
Presidente - 1173660 - FABIO BARBOUR SCOTT
Interno - 124.088.737-03 - DIEFREY RIBEIRO CAMPOS - UFRRJ
Interna - 1700427 - YARA PELUSO CID
Externo à Instituição - GUILHERME MARCONDES KLAFKE
Externo à Instituição - ISABELLA VILHENA FREIRE MARTINS - UFES