Banca de DEFESA: CARLA JULIETE DOS REIS SARDELLA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : CARLA JULIETE DOS REIS SARDELLA
DATE: 08/04/2022
TIME: 09:30
LOCAL: Plataforma Google Meet
TITLE:

Nematodes of the genus Contracaecum (Anisakidae) parasitic in Nannopterum brasilianus (Suliformes: Phalacrocoracidae) from Argentina: Morphlogical and molecular diagnostic of larval and adult specimens.

KEY WORDS:

Contracaecum; larval; adults; Cox2; scanning electron microscopy; Hoplias

PAGES: 60
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:

Contracaecum Raillet & Henry, 1912 is a genus of parasitic nematodes of the Anisakidae family Skrjabin & Karokhin, 1945. These nematodes are parasites of fish-eating animals, i.e., mammals and birds, from where they acquire third-stage larvae that develop into sexually mature adults. The eggs and successive stages of their larvae use invertebrates and some classes of fish as intermediate hosts. This is the only genus of the Anisakidae family that can infect terrestrial, marine and freshwater animals. Individuals of the Contracaecum genus are characterized by presenting an esophagus with a ventricle, presence of posterior ventricular appendix, anterior cecum, excretory pore located at the level of the lips and adults presenting interlabia. The present work aims to contribute with knowledge about the biodiversity of fish and bird parasitic nematodes of the Contracaecum genus in Argentine waters, adding new molecular and morphological data. In chapter 1, the objective was to describe a new species of Contracaecum using adults and fourth-stage larvae (L4) collected from Nannopterum brasilianus (Gmelin, 1789) (syn. Phalacrocorax brasilianus) (Suliformes: Phalacrocoracidae) and third-stage larvae (L3) found in freshwater fish Hoplias argentinensis Rosso, Mabragaña, González-Castro, Bogan, Cardoso, Mabragaña, Delpiani & Díaz de Astarloa, 2018, both from the province of Córdoba, Argentina, using morphological approaches such as light and scanning electron microscopy (SEM) and molecular based on the sequencing of the mitochondrial gene of cytochrome c oxidase subunit II (cox-2), which allowed us to relate the larvae found in fish with the adults found in birds. Chapter 2, on the other hand, aimed to describe a new species based on morphological characteristics of the fourth larval stage and adults of a new species also from the genus Contracaecum, found parasitizing the bird N. brasilianus in an Argentine lake. It was also possible to relate the larvae and adults found in the bird based on morphometry. So far there is a record of 5 species of parasites of the genus found in N. brasilianus in the American continent, totaling 7 with the inclusion of these two new species, Contracaecum jorgei and Contracaecum n. sp. showing the importance of studies related to the life cycle of these parasites, especially those in which the larvae are found in fish intended for human consumption, as the high density of fish recorded in Pampa lakes in Argentina can increase the food supply for the birds, improving the chance of infecting the definitive host, allowing the cycle to continue and eventually infecting humans who consume raw fish containing the L3 of Contracaecum.

BANKING MEMBERS:
Presidente - 2242796 - JOSE LUIS FERNANDO LUQUE ALEJOS
Externa ao Programa - 3092534 - RAQUEL DE OLIVEIRA SIMOES
Externo à Instituição - ANDERSON DIAS CÉZAR - UES
Externo à Instituição - DIMITRI RAMOS ALVES - UNIFOA
Externo à Instituição - FABIANO PASCHOAL DE OLIVEIRA - UCB