Banca de DEFESA: EMILY ANDRESSA SANTOS LIMA
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : EMILY ANDRESSA SANTOS LIMA
DATE: 10/08/2022
TIME: 14:00
LOCAL: Plataforma Zoom
TITLE:
Insecticidal and repellent activity of the essential oils of Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea against the immature and adult stages of Ctenocephalides felis felis.
KEY WORDS:
Flea; Copaiba; Lavandin; Sage; Grapefruit.
PAGES: 105
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUBÁREA: Medicina Veterinária Preventiva
SPECIALTY: Doenças Parasitárias de Animais
SUMMARY:
The flea Ctenocephalides felis (Siphonaptera: Pulicidae) is a parasitic insect of dogs and cats with a cosmopolitan distribution. They are insects of importance in public health, they stand out for being vectors of several pathogenic agents to animals and man. The number of research and tutors looking for products of plant origin, less toxic and causing less damage to the environment, is growing. The aim of this research was to determine the chemical composition of the essential oils of Copaifera reticulata, Citrus paradisi, Lavandula hybrida and Salvia sclarea, evaluate the insecticidal activity, repellent action, calculate the lethal concentrations 50 (LC50) and 90 (LC90) and residual efficacy against to the flea Ctenocephalides felis felis. Chemical composition analysis was performed by Gas Chromatography (GC) equipped with a flame ionization detector (FID), and a split/split-less injector to separate and detect the volatile constituents of essential oils. For the in vitro test, a stock solution of essential oils (EO's) diluted in 20% acetone was prepared at different concentrations. To evaluate the insecticidal activity, ten specimens of each stage (egg, larva, pupa and adults) were used. To evaluate the inhibition of the biological cycle, ten eggs were used, and, for the residual efficacy test, ten adult fleas were used. All tests were performed in six repetitions. The adult phase was placed in test tubes (1x10 cm), and a strip of impregnated filter paper was inserted with the concentrations of each essential oil. The tests in the immature phase, a petri dish (60x15mm) was used, and inserted into a filter paper disc impregnated with the same concentrations. The number of live and dead insects after a given time of exposure was recorded. We used the following formula to calculate the mortality percentage: mortality (%) = number of dead insects X 100/number of incubated insects. For statistical evaluation, the tabulated data were operated, and, for the Probit analysis, the computer program R Studio Team software was used to calculate the LC50 values with a confidence interval of 95%. The constituents with the highest percentage found were: β-caryophyllene for C. reticulata EO (OECR), linalool for L. hybrida (OELH), linalyl acetate for S. sclarea (OESS) and limonene for C. paradisi (OECP). Regarding the insecticidal activity, it was possible to obtain the LC50 for the stages of adult, egg, larva, pupa and inhibition of the development of all OE`s, except for the OECP. For the repellent action, all oils showed activity for the concentration of 800 µg/cm2. Regarding the persistence of efficacy, it was possible to observe that the OECR presented greater persistence of efficacy, when compared to the others.It is concluded that each tested EO presents a better insecticidal activity for each phase of C. felis felis.
BANKING MEMBERS:
Presidente - 2456316 - KATHERINA COUMENDOUROS
Interna - 2929889 - THAIS RIBEIRO CORREIA AZEVEDO
Interna - 1700427 - YARA PELUSO CID
Externo à Instituição - FERNANDO HENRIQUE CORRER
Externo à Instituição - ISABELLA VILHENA FREIRE MARTINS - UFES