Banca de QUALIFICAÇÃO: JOSÉ PAULO DO CARMO SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : JOSÉ PAULO DO CARMO SILVA
DATA : 26/09/2019
HORA: 14:00
LOCAL: Sala 34 do ICBS
TÍTULO:

The use of morphometry and microchemistry of the saggitae otolits and genetic markers as tools to investigate the population connectivity of Mugil liza (Valenciennes, 1836) on the the southeastern coast of Brazil

PALAVRAS-CHAVES:

Connectivity; fishery resources; otoliths; molecular markers; coastal zones

PÁGINAS: 75
GRANDE ÁREA: Ciências Biológicas
ÁREA: Ecologia
SUBÁREA: Ecologia de Ecossistemas
RESUMO:

The study aims to investigate the connectivity of an important fishery resource along the
southeastern Brazilian coast of (mullet, Mugil liza) between semi-closed coastal systems
and the adjacent coastal zone, aiming to provide subsidies for preservation and
sustainable use. Three approaches are used to achieve this objective, which correspond
to the three chapters of the Thesis: 1. Sagittae otolith morphometry; 2. Sagittae otolith
biochemistry; 3) genetic markers. The fishes were collected along about 612 km,
covering the coast of the states of Rio de Jneiro and São Paulo, in the following semi-
closed systems and in their respective adjacent coastal zones (Lagooons of

Saquaremma, Maricá and Santos/ Estuary), in the bays of Guanabara, Sepetiba and Ilha
Grande, and near the coast of Caraguatatuba and Ilha Bela. We investigate, through the
three approaches, the existence of different population groups throughout the study area,
the population connectivity between the systems and the stocks/populations and the
existence of different movement patterns of the individuals within the different systems
(lagoons, bays and estuaries). 916/5000
In the first chapter we tested the existence of different stocks of mullets along the coast
of the states of São Paulo and Rio de Janeiro. Assuming that different morphotypes
and/or variations in otolith shape may represent different populations/stocks, we tested
the hypothesis that there is more than one Mugil liza stock/population across the study
area. For this, we used Fourier elliptical descriptors and morphometric indices to
identify variations in the shape of otoliths throughout the study area. A geographical
pattern of separation, suggesting the existence of at least two stocks/populations
throughout the study area was detected, and the presence of three otolith morphotypes
that did not show a clinal distribution pattern was found, suggesting the study area as a
transition area for different stocks / populations of M. liza. In the second chapter, the
patterns of movement and use of semi-closed systems and open coastal zone were
studied. Assuming that mullets use estuarine systems during the early phase of their life
cycle and migrate to the platform as adults, making up the migratory/spawning stock of
the species. We tested the hypothesis that there is an individual differentiation of use,
where some individuals return to the estuarine environment after spawning and some
individuals do not need to exit estuarine systems to complete their life cycle. For this,
the elemental strontium and barium profiles of the mullets captured within the main
estuarine systems and respective coastal zone sites were evaluated. Of all core-edge axis
only Sr was different between sites, with Magé having a higher concentration than the
other sites. The same occurred for the edge of the otoliths, but for the nucleus Sr/Ca was
larger in Ilha Grande, followed by Magé, Sepetiba Bay and Guarujá. Ba was not
different in the nucleus-edge axis or in the otolith edge, however, in the nucleus there
was a difference with higher concentrations in the Maricá Lagoon than in Itaipu,
Guarujá Fora and Ilha Bela Fora. An acceptable rating for Saquarema (54%), Magé
(50%) and Maricá (66.6%) locations was found, whereas the others (Sepetiba Bay,
Guarujá and Caraguatatuba) were below 50%. In the third chapter, we test the existence
of different stocks of mullets along the coast of the states of São Paulo and Rio de
Janeiro. Assuming that the geographical distance between the analyzed extreme points

may represent, in part, a barrier to the dispersal of genes of the species. Thus, we tested
the hypothesis that there is more than one Mugil liza stock/population across the study
area. DNA extraction was performed from 133 individuals. The amplification reaction
(PCR) of the 09 selected primers was successful for all samples. Sample genotyping has
already been performed and genotyping data output is currently being spread out for
sequence in data analysis.

MEMBROS DA BANCA:
Externo à Instituição - ALBERT LUIZ SUHETT
Interno - 387200 - FRANCISCO GERSON ARAUJO
Interno - 3138877 - RICARDO UTSUNOMIA
Externo à Instituição - TAYNARA PONTES FRANCO