Banca de DEFESA: DIEGO MATTOS PENEDO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : DIEGO MATTOS PENEDO
DATE: 30/07/2020
TIME: 09:00
LOCAL: ON-LINE
TITLE:

Phenotypic, cytogenetic and molecular genetic analysis in black-horned capuchin, Sapajus nigritus (Platyrrhini, Cebidae) from areas of Rio de Janeiro and São Paulo states, Brazil

KEY WORDS:

Philogeography; Constitutive heterochromatin; Mitochondrial genes

PAGES: 94
BIG AREA: Ciências Biológicas
AREA: Genética
SUBÁREA: Genética Animal
SUMMARY:

The black-horned capuchin, Sapajus nigritus, is a Neotropical primate with a wide distribution in the Atlantic Forest (AF), from southeast Brazil to Misiones, Argentina. The species presents phenotypic and genetic diversity, often associated with geographical patterns. The coat coloration varies with intrapopulation differences, with transition in the predominance from black to brown, in the south-north distribution. Cytogenetic analysis in populations from Argentina showed a specific polymorphism in the chromosome pair 11. While in most Sapajus’ species this pair is the largest acrocentric, with a terminal extracentromeric heterochromatic (HC) block, in S. nigritus in these populations the total deletion of HC is described. However, the terminal He block has already been demonstrated in specimens from RJ. In addition to this polymorphism, phylogenetic data demonstrate paraphyly among samples from RJ and other localities along the distribution. These variations may correspond to a pattern of phylogeographic discontinuity described in AF at SP, associated to forest refuges occurring in Pleistocene. The objective of this study was to analyze the phenotypic and genetic diversity of S. nigritus in SP and RJ, investigating the coat patterns, the distribution of He in the par 11 and the molecular diversity in the state of RJ, comparing it with other localities. Individuals from five populations in the RJ state were analyzed: Ilha da Marambaia (IM), Reserva Biológica de Poço das Antas (RBPA), Parque Nacional do Itatiaia (PNI), da Tijuca (PNT) and da Serra dos Órgãos (PARNASO); as well as ex situ specimens from Guaratiba/RJ (Gua) and cities of São Paulo: capital (cSP), São José dos Campos (SJC), São Sebastião (SS), Paraibuna (PB) and Campos do Jordão (CJ). Specimens of S. nigritus at the Museu Nacional do Rio de Janeiro (MNRJ), from Angra dos Reis, Itatiaia, Paraty and Teresópolis, were included in the phenotypic analysis. Coloration of the back, belly, limbs, tail and face were evaluated. The presence and distribution of He were analyzed using classical and molecular cytogenetic techniques. The phylogenetic relationships were inferred based on the mitochondrial genes cytochrome c oxidase I, II and cytochrome b, including sequences available in GenBank®. In 49 individuals captured, six ex situ and eight MNRJ specimens were identified brown (n=37) and black (n=26) coat patterns, with intrapopulation variation, predominating black to the west (PNI and MNRJ) and brown to the east (IM and PNT). The association of this finding with the predominance of the black pattern described in an SP population confirms the black-brown variation in the SP-RJ distribution. The pair 11 with the deletion of HC was identified in the samples to the east (IM, Gua, PNT, PARNASO and RBPA), while to the west both patterns were identified, without (cSP, CJ and SS) and with (SJC, PB and PNI) He block. Molecular divergence was observed between the samples from RJ and those from SP, PR and Argentina, including the PNI, forming paraphyletic clades. The intraspecific diversity observed in S. nigritus may represent an evolutionary significant unit (ESU) and confirm the sampled region as a phylogeographic discontinuity point for the species.

BANKING MEMBERS:
Externa à Instituição - MARIELA NIEVES
Externa à Instituição - ANDRÉA MARIA DE OLIVEIRA
Externo à Instituição - RODRIGO SALLES DE CARVALHO
Externo à Instituição - CARLOS EDUARDO DA SILVA VERONA
Interna - 1539011 - DENISE MONNERAT NOGUEIRA
Externa à Instituição - LENA GEISE