Banca de DEFESA: CARLOS AUGUSTO DOS SANTOS SOUSA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : CARLOS AUGUSTO DOS SANTOS SOUSA
DATE: 09/08/2021
TIME: 14:00
LOCAL: on-line
TITLE:

COMPARATIVE MORPHOLOGICAL STUDY OF THE PENIS OF THE PAMPA FOX (Lycalopex gymnocercus, Fischer 1814) AND CRAB-EATING FOX (Cerdocyon thous, Linnaeus 1766)

KEY WORDS:

Canids, histoarchitecture, copulatory organ

PAGES: 64
BIG AREA: Ciências Biológicas
AREA: Zoologia
SUBÁREA: Morfologia dos Grupos Recentes
SUMMARY:

Although canids are from a very heterogeneous family, they can be commonly separated into two distinct types: the dogs, wolves and jackals and the foxes and graxains. As important species in the modulation of the structuration of biological communities, they are frequently found in breeders and zoos. The aim of this study was to characterize the morphology of the penis of C. thous and L. gymnocercus in order to provide information that can help the reproductive management in captivity and favor the clinical conduct involving this organ as well as to contribute to comparative animal morphology. For this, fourteen cadavers, males and adults (seven C. thous and seven L. gymnocercus) were used. After the animals were placed in the right lateral decubitus position, and their chest wall was dissected to access to the thoracic aorta, which was properly cannulated for fixation with a 10% formaldehyde solution. Afterwards, the animals were dissected and anatomical regions, as well as the static structures of the penis of both species, were registered and identified. For the macroscopic characterization, two penises from each animal were cut at different regions for examination under a stereoscopic microscope: a sagittal and a transverse section. The remaining ten penises from cleaved region of the penis body (5 of each species) were subjected to routine histological technique for embedding in paraffin. From each sample, 5 slices with 5 micrometers of thickness each fragment were used and stained with hematoxylin-eosin, Mallory’s trichrome, Orcein and Picrosirius red method. The smooth muscle cells were ideitify by immunohistochemistry using anti-alpha-actin antibody. The results revealed that pênis of both species possess a fibroelastic histoarchitecture in contrast to described in the current literature for carnivores. As the anatomical regions of the penises, root, body and glans were identified. Penile areas (total penile area, corpus cavernosum area, corpus spongiosum area and luminal area of the urethra) were measured. The luminal area of the urethra of C. thous and L. gymnocercus measured 0.089 ± 0.048, and 0.095 ± 0.037, respectively (P = 0.8177). Total area of the penis in C. thous was 26.981 ± 4.842 while in L. gymnocercus was 20.228 ± 5.245 (P = 0.0673). In addition, the corpus cavernosum area of C. thous was significantly larger (9.929 ± 2.607) than that of L. gymnocercus (6.083 ± 1.048) (P = 0.0156). The corpus spongiosum area of L. gymnocercus was significantly larger (2.068 ± 0.424) when compared to C. thous (1.520 ± 0.188) (P = 0.0297). The histochemical and immunohistochemical results revealed a fibroelastic histoarchitecture, in contrast to descritions observed in the current literature for carnivores Thus, this research can contribute to the understanding of the reproductive behavior of these carnivores, in addition to helping to improve the management of animals in captivity, as well as contributing to improve clinical conduct in supporting the diagnosis of penile diseases

BANKING MEMBERS:
Externo à Instituição - PAULO SOUZA-JÚNIOR - Unipampa
Externo à Instituição - YURI KARACCAS DE CARVALHO - UFAC
Externo à Instituição - ROGÉRIO MAGNO DO VALE BARRO - UFAC
Externo à Instituição - ANDRÉ RODRIGUES BARRETO DA CUNHA VIANNA - UFPR
Externa ao Programa - 2142679 - LEILANE MARIA BARCELLOS NEPOMUCENO
Presidente - 2181722 - MARCELO ABIDU FIGUEIREDO