Banca de QUALIFICAÇÃO: WELLINGTON THADEU DE ALCANTARA AZEVEDO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : WELLINGTON THADEU DE ALCANTARA AZEVEDO
DATE: 14/09/2023
TIME: 14:00
LOCAL: on-line
TITLE:

Study of the Dipterofauna (Calliphoridae and Mesembrinellidae) in the Três Picos State Park, Rio de Janeiro: applications in environmental impact assessment and Forensic Entomology

KEY WORDS:

Bioindicators, Environmental gradient, Edge effect

PAGES: 67
BIG AREA: Ciências Biológicas
AREA: Zoologia
SUBÁREA: Zoologia Aplicada
SUMMARY:

The Atlantic Forest is a biome that encompasses forests, sandbanks, mangroves and
high-altitude fields, with a rich biodiversity, including more than 15,000 species of
endemic vascular plants. It is under constant pressure due to urbanization and extraction
of natural resources, resulting in the fragmentation of its habitat, which leads to the edge
effect, causing changes in biotic and abiotic conditions in the marginal areas of the
remnants, affecting the dispersal of species and leading to the loss of biodiversity. The
Parque Estadual dos Três Picos is a full protection area created in 2002 to preserve the
Atlantic Forest. This study aims to study the fauna of Calliphoridae and
Mesembrinellidae in the Parque Estadual dos Três Picos, municipality of Cachoeiras de
Macacu, state of Rio de Janeiro and identify bioindicators to evaluate the degree of
preservation of the area, considering anthropogenic influences and abiotic factors. The
edge effect was evaluated at five sampling points along a gradient of 800 meters from
the edge of the park towards the interior of the forest. Two traps were installed at each
sampling point with fresh beef liver bait. To study the synanthropy and attractiveness of
the bait by different stages of decomposition, only the innermost point of the forest
environment was considered, to which two traps containing bovine liver bait with 48
hours of decomposition were added, and two additional collection points were included:
a rural environment on the campus of Seropédica, Universidade Federal Rural do Rio de
Janeiro and an urban area in the Instituto de Biociências, Universidade Federal do
Estado do Rio de Janeiro. Collections were conducted quarterly over a two-year period,
from June 2021 to May 2023. After collection, the specimens were sacrificed using a
solution of ethyl alcohol and ethyl acetate and transferred to properly identified
containers. Taxonomic identification was performed based on the observation of
morphological characters under stereoscopic microscope and consultation of taxonomic
descriptions and keys. The materials were destined for the entomological collections of
the Universidade Federal do Rio de Janeiro and Universidade Federal do Estado do Rio
de Janeiro. A total of 5,476 dipterans were collected, being 5 species of Calliphoridae
and 11 of Mesembrinellidae. The Calliphoridae family represented 77.1% of the total
collected, the most abundant species were Lucilia eximia (59.3%) and Hemilucilia
segmentaria (12.9%). The Mesembrinellidae family contributed with 22.9%, with the
most abundant species being Mesembrinella bellardiana (16.3%) and Laneella nigripes
(4.5%). Seven species occurred commonly, one occurred rarely, five were constant, and
seven were accidental. Huascaromusca aneiventris and Paralucilia nigrofacialis
showed a preference for environments with greater canopy coverage. Species
Eumesembrinella cyaneicyncta and Hemilucilia benoisti were more related to the points
at 0 and 200 meters. Laneella nigripes showed an increase in its abundance at the points
farthest from the edge. Mesembrinella bellardiana was more abundant at the
intermediate points. Hemilucilia segmentaria and Lucilia eximia were abundant along
the entire gradient. Mesembrinella semihyalina was negatively correlated with plant
density, and Hemilucilia benoisti was negatively correlated with canopy cover. Lucilia 

eximia, Hemilucilia segmentaria, Hemilucilia semidiaphana, Hemilucilia benoisti,
Paralucilia nigrofacialis, Mesembrinella bellardiana and Mesembrinella peregrina
correlated with precipitation, temperature and/or relative humidity. The diversity profile
of Rényi varied throughout the collection period, with greater richness and diversity in
winter and lower in autumn. The analysis of the species in different ecological areas
revealed the species of Mesembrinellidae occurring exclusively in the forest
environment, in addition to Hemilucilia benoisti and Paralucilia nigrofacialis, which
were classified as sign-tropic. Hemilucilia segmentaria and Hemilucilia semidiaphana
were also classified as signanthropic, but occurred in urban and/or rural environments.
The species of Chrysomya and Cochliomyia, and Lucilia cuprina, were considered
synanthropic. The study identified eight species with the potential to serve as
bioindicators in forest environments, especially Laneella nigripes, Mesembrinella
bellardiana and Lucilia eximia, and four species for the rural environment, with
emphasis on Cochliomyia macellaria. Only Laneella nigripes showed a preference for
liver baits with 48 hours of putrefaction. These results provide valuable information
about the ecology and distribution of the dipteran species Calliphoridae and
Mesembrinellidae in different environments, as well as their potential as environmental
indicators and their behavior in relation to the stage of bait decomposition. These
findings may contribute to conservation studies and environmental monitoring in the
region studied. The Três Picos State Park is a well-preserved environment.

COMMITTEE MEMBERS:
Externa à Instituição - VALÉRIA CID MAIA - UFRJ
Interno - 387200 - FRANCISCO GERSON ARAUJO
Interno - ***.893.247-** - JERONIMO AUGUSTO FONSECA ALENCAR - FIOCRUZ