Banca de QUALIFICAÇÃO: ELVIS ALMEIDA PEREIRA SILVA

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : ELVIS ALMEIDA PEREIRA SILVA
DATA : 13/12/2019
HORA: 10:00
LOCAL: ICBS
TÍTULO:

Diversification Patterns and Processes in Amazon and Atlantic Forest Amphibians

PALAVRAS-CHAVES:

Atlantic Forest, Amazon, Caatinga, Cerrado, Approximate Bayesian Computation, Coalescent Methods, Diversification

PÁGINAS: 70
GRANDE ÁREA: Ciências Biológicas
ÁREA: Zoologia
SUBÁREA: Taxonomia dos Grupos Recentes
RESUMO:

Since the emergence of phylogeography, much data has been generated on the
patterns of distribution of populations of Atlantic Forest organisms. Some
phylogeographic breaks were prominent, such as those existing in the São Francisco and
Rio Doce basin, as well as a break in the southern region of the biome. Studies using
climate paleomodels to infer past distribution of Atlantic Forest taxons have incorporated
a predictive component to phylogeographic studies in Brazil. In addition, they provided
scenarios for testing the hypotheses of demographic expansion of Atlantic Forest
organisms associated with the Pleistocene glacial and interglacial periods. When
investigating the demographic history of taxa that currently inhabit cold and altitude
areas, demographic expansion was observed during the Pleistocene. Already the
organisms that inhabit lowland areas, it was observed that they retracted their distribution
during the Pleistocene, and presented demographic expansion from north to south after
the end of the last glacier. The Atlantic Forest, a highly diverse forest formation
threatened by anthropic action, is the focus of interest in several studies on species
diversification. The aim of this study is to reconstruct, through a wide geographical
approach, the evolutionary history of a taxon distributed in the Atlantic Biome that enters
the Cerrado Biome. Methods based on Bayesian inference and coalescence theory were
used to 1) identify the main lines of Boana crepitans species, 2) to estimate the divergence
time between them and 3) to investigate the existence of possible cryptic species.
Phylogeographic reconstruction techniques, historical demography and niche modeling
were employed in the search for additional evidence to the processes involved in taxon
diversification. The obtained results bring new information for the understanding of
biogeography, as well as clarifications to the taxonomy of B. crepitans. The 16S
mitochondrial marker was sequenced for 116 samples and 37 sequences of the 12S and
COI markers were imported from GenBank as well as the proopiomelanocortin nuclear

markers (POMC) and recombinant activation 1 (RAG1) were sequenced for 52 tissue
samples of B. crepitans, much of its geographical distribution. Based on the
mitochondrial tree lineages, we made the population mark. We calculated summary
statistics and genetic divergence between lineages. Boana crepitans presented divergence
in mtDNA and nuDNA, with high structure of two strains. One strain grouped specimens
from northern Minas Gerais, southern Bahia and Goiás and Tocantins states, while the
second grouped the other localities of the Brazilian coast. Both approaches of population
marking were congruent with mitochondrial lineages, estimating two populations. In
both, nucleotide diversity was low. The genetic distance within populations was between
91 and 98%, while the distance between populations was between 1 and 9%. Gene flow
reduction and isolation are directly related to species diversification.

MEMBROS DA BANCA:
Presidente - 387200 - FRANCISCO GERSON ARAUJO
Interno - 3138877 - RICARDO UTSUNOMIA
Interno - 2289640 - HELIO RICARDO DA SILVA
Interno - 1539011 - DENISE MONNERAT NOGUEIRA