Banca de DEFESA: LUAN VALIM DOS SANTOS

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUAN VALIM DOS SANTOS
DATE: 14/12/2020
TIME: 13:00
LOCAL: on-line
TITLE:

Diferencial expression of brummer and levels of TAG in different developmental stages and starvation of Aedes aegypti (Diptera: Culicidae)

KEY WORDS:

larvae, fat body, lipid storage

PAGES: 67
BIG AREA: Ciências Biológicas
AREA: Zoologia
SUBÁREA: Zoologia Aplicada
SUMMARY:

Lipid storage in the form of triacylglycerol (TAG) is essential during the
insect life cycle, as it enables body transformation during the pupal stage and supports
energy expenditure during the early phase of adult life and reproductive maturation. The
lipolytic activity of the brummer (bmm) lipase facilitates the insect energy system
homeostasis. We observed the relationship between bmm lipase and lipid storage
in Aedes aegypti larvae and adults. The bmm sequence was identified in A. aegypti,
exhibiting a patatin-like phospholipase domain, reinforced by the presence of a catalytic
dyad with serine and aspartate residues, revealing a high degree of similarity with other
organisms. The bmm expression was differentiated in the larval phase and the adult
animal fat bodies, following the TAG reserve dynamic. In larval stages L3, L4, and
pupa, Bmm was expressed three times as much compared to stages L1 and L2. In the
post-emergence (PE) and post-blood-meal (PBM) fat body of adult animals, the
expression varied over several days. When post-emergence adult animals fasted,
without sucrose, a very pronounced increase was observed from the third day onwards
when compared to animals that did not fast. A significant decrease in the levels of TAG
from the third day, which suggests Brummer’s participation in the process. Six hours
after blood feeding, the levels of TAG increased suggesting a process of lipid reserve
accumulation to guarantee oogenesis and reproduction. The bmm in A. aegypti responds
positively to fasting and regulates the mobilization of TAG reserves in adult
mosquitoes' fat bodies.

BANKING MEMBERS:
Interno - 1555317 - EMERSON GUEDES PONTES
Interno - 005.893.247-05 - JERONIMO AUGUSTO FONSECA ALENCAR - UFRJ
Externa ao Programa - 2860277 - ISABELE DA COSTA ANGELO
Externo à Instituição - LUCIANO APARECIDO MEIRELES GRILLO
Externo à Instituição - MARCO ANDRE ALVES DE SOUZA