Banca de DEFESA: MARIA LUIZA RIBEIRO DE OLIVEIRA

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
DISCENTE : MARIA LUIZA RIBEIRO DE OLIVEIRA
DATA : 04/03/2019
HORA: 09:30
LOCAL: sala 34 do ICBS
TÍTULO:

Reproductive compatibility and morphological characterization of sperm in species of the genus Triatoma (Hemiptera, Reduviidae, Triatominae)

PALAVRAS-CHAVES:

Triatoma rubrovaria subcomplex, insect vector, reproduction, male reproductive system

PÁGINAS: 85
GRANDE ÁREA: Ciências Biológicas
ÁREA: Parasitologia
SUBÁREA: Entomologia e Malacologia de Parasitos e Vetores
RESUMO:

Spermatozoa are among the most diverse cell types, and their morphologies often provide
data that can be used to reliably evaluate phylogenetic relationships. They can also help to
clarify the nature of “specific complexes”, which are common among triatomines. In the
present study, we evaluated the copulation behavior of Triatoma rubrovaria and the structural
morphology of sperm from T. carcavalloi, T. infestans, T. pintodiasi, and T. rubrovaria.
Copulatory behavior was described from the moment males and females genitalia joined until
they separated. Insemination was confirmed by the presence of a spermatophore in the
female’s bursa copulatrix. To measure their sperm, males were dissected and their seminal
vesicles were removed, squashed on glass slides, and then spread, fixed, and observed under a
photomicroscope. The images obtained were analyzed to measure the sperm. Seminal vesicles
were also prepared for transmission electron microscopy. We performed K-means clustering
separately for each species to group their sperm based on morphology. The differences in
spermatozoa length among species of Triatominae, sperm types, and the interaction between
species and sperm type were assessed with a two-way analysis of variance (ANOVA). The
copulation time in T. rubrovaria was 3 to 5 min, which was sufficiently long for
spermatophore transfer. All taxa showed polymorphic (short and long) sperm, with significant
differences in the lengths of sperm among taxa. Using electron microscopy, the sperm cells of
the four taxa examined were found to have similar ultrastructural morphology, confirming the
hypothesized synapomorphies of sperm within the suborder Heteroptera

MEMBROS DA BANCA:
Externo à Instituição - ALICE RICARDO DA SILVA
Externo à Instituição - ANGELA ALVES DE ALMEIDA
Externo à Instituição - CLEBER GALVÃO
Externo à Instituição - ELIDIOMAR RIBEIRO DA SILVA - UFRJ
Presidente - 710.008.957-34 - JACENIR REIS DOS SANTOS MALLET - FIOCRUZ
Externo à Instituição - JERONIMO AUGUSTO FONSECA ALENCAR