Banca de DEFESA: DIOGO PIGNATARA COIMBRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : DIOGO PIGNATARA COIMBRA
DATE: 31/07/2020
TIME: 09:00
LOCAL: ON-LINE
TITLE:

Analysis of hemoparasites in Callithrix (primates: callitrichidae) individuals from free living and captivity in the metropolitan region of Rio de Janeiro, Brazil

KEY WORDS:

parasitology, microscopy, molecular genetics

PAGES: 113
BIG AREA: Ciências Biológicas
AREA: Genética
SUBÁREA: Genética Animal
SUMMARY:

Neotropical primates are hosts of various hemoparasites species. Callithrix marmosets inhabiting the Rio de Janeiro metropolitan region interact with humans and can share possible zoonoses. The objectives of this study are: to identify at the species level hemoparasites of the marmosets of the metropolitan region of Rio de Janeiro through optical microscopy, morphometry and molecular genetics; estimate and compare the epidemiological values of prevalence and parasite mean intensity between in situ and ex situ localitions. Two collections were carried out in two in situ locations, Jardim Botânico do Rio de Janeiro (JBRJ) Universidade Federal Rural do Rio de Janeiro (UFRRJ), and two ex situ localitions, Biotério of the Universidade Federal do Rio de Janeiro (Biotério-UFRJ) and Wild Animals Triage Center of Rio de Janeiro (CETAS-RJ). Samples were also analyzed from the Wild Animals Recovery Center of the Universdade of Vale do Paraíba in São Paulo (CRAS-UNIVAP) as a control site. Animals captured were sedated and about 1 ml of blood was collected for blood smears and molecular genetic analysis. The slides were analyzed under a microscope with photographic record and morphometric measurements. Prevalence and mean parasite intensity were analyzed. DNA was extracted from the blood samples using the phenol-chloroform method. Primers were used for amplification of the 18S rRNA gene of Trypanosoma and ITS-1 for microfilaria by the polymerase chain reaction. The 18S rRNA gene amplicons were sequenced, aligned by BLAST® and submitted to phylogenetic analysis. It was found in the JBRJ samples Trypanosoma sp., Microfilaria and Babesia sp. In the UFRRJ and CETAS-RJ samples, Trypanosoma sp. Through the morphometric analysis, the Trypanosoma of the JBRJ and UFRRJ were identified as T. minasense with measures in the range expected for species in characters TL 28.4 and 48, PK 6.8 - 15 and B 2 - 6 μm. The amplified fragments for Trypanosoma presented 700 bp as the positive control, and the microfilaria fragments presented between 200 and 300 bp as in the literature. Sequencing of the PCR amplified fragments of the Trypanosoma 18S rRNA gene revealed genetic similarity above 98% with T. minasense, grouping in the same clade in the phylogenetic analysis. The morphometric data suggest that the microfilariae found are Mansonella marmosetae and Dipetalonema graciliformis, being possible the presence of more species. Through microscopy, in situ localities presented infection rate of 25% for Trypanosoma sp., 11% for microfilariae and 3% for Babesia sp. Ex situ localities presented infection rate of 2% for Trypanosoma sp. With molecular genetic data, the infection rate of Trypanosoma in situ was 43% against 14% in ex situ localities, on the other hand microfilariae was 21% and 44%, respectively. This result partially corroborates the hypothesis that in situ localities are more likely to have hemoparasitoses due to the diversity and availability of niches, contact with other specimens and to lower factors of physical impediment of the vectors. Differences regarding the microfilaria infection rates should be related to the density of hosts and the limitation of their movements.

BANKING MEMBERS:
Interna - 1539011 - DENISE MONNERAT NOGUEIRA
Externo ao Programa - 386276 - JORGE LUIS AZEVEDO DE ARMADA
Externa ao Programa - 3103478 - MARISTELA PECKLE PEIXOTO
Externo à Instituição - CARLOS EDUARDO DA SILVA VERONA
Externa à Instituição - CLAUDIA BEZERRA DA SILVA