Banca de QUALIFICAÇÃO: AMANDA QUEIROZ BASTOS

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : AMANDA QUEIROZ BASTOS
DATE: 27/07/2020
TIME: 10:00
LOCAL: ON-LINE
TITLE:

Bionomy of culicidae and natural investigation for flavivirus in a landscape of the Atlantic Forest biome in the state of Rio de Janeiro, Brazil

KEY WORDS:

Culicidae; diversity; natural infection, zika

PAGES: 79
BIG AREA: Ciências Biológicas
AREA: Parasitologia
SUBÁREA: Entomologia e Malacologia de Parasitos e Vetores
SUMMARY:

The mosquitoes, family Culicidae, have 3,568 species classified in two subfamilies:
Anophelinae and Culicinae. Both have species of epidemiological importance. The fauna
inventory of mosquitoes in areas of natural environment is condiderably important in ecological
studies. The natural populations of animals in a given biogeographic area distributed in
communities subject to constant fluctuations in response to the environment. During the
processes of anthropic action or in the possible periods of recovery of the vegetation cover,
knowledge of the biodiversity of mosquito communities in na Atlantic Forest environment is of
relevant importance. Since this knowledge is useful to evaluate possible changes in behavior
and adaptations in the pattern of activities of mosquitoes considered to be, until then, of wild
habit, preferably. The sampling resulted from collections made in two fragments of Atlantic
Forest in the state of Rio de Janeiro in municipality of Nova Iguaçu in the Associação de
Taifeiros da Armada (ATA) and in Boa Esperança site. The duration of collections in both
locations was one year.The present study aimed to assess the richness, diversity, equitability and
preference of immatures and adults in both fragments and the influence of climatic factors
(temperature, relative humidity and rainfall) on the abundance of mosquitoes. In the two study
areas, biweekly visits, sytematic and standardized sampling were performed using a manual
sucker to collect the larvae. In ATA, the natural breeding sites surveyed were bamboo stalks,
while in the Boa Esperança site, ovitraps were randomly distributed and the natural bamboo and
bromeliad breeding sites were monitored. Adult spcimens were captured using CDC light traps
with CO2 attractiveness, part of the specimens from the collected eggs were used to perform the
analysis of possible natural infection by flavivirus (Zika, yellow fever). During the sampling
period in the ATA, 3170 larvae were collected in perforated bamboo in eight stems of Five
plants, of which 688 specimens reached the adult stage, distributed in 10 genera and 19 species.
The richness of the immatures collected in the ATA was 20 species, with a recorded diversity of
1.817 and Shannon’s equitability of 0. 6064. In the sample area of the Boa Esperança site, 5514
larvae were collected and 1322 reached the adult stage: 949 in the plastic container, 80 in the
bromeliad and 293 in the bamboo. Of the 5868 eggs collected, 3991 hatched, and 3807 reached
the pupal stage and 2420 reached the adult stage. The species from the eggs with the greatest
abundance were Aedes albopictus with 61% and Haemagogus leucocelaenus 34%. In the CDC
light trap with CO2 attractiveness, 2621 specimens of mosquitoes of these genera were: Culex
(84,9%), Wyeomyia (5,3%), Aedes (3,5%) and Limatus (3,1%). A total of 1,229 specimens of
mosquitoes: Ae. albopictus (651), Hg. leucocelaenus (565) and Hg. janthinomys (13) from eggs
collected at the Boa Esperança site were submitted to RT-PCR to detect flavivírus. The bamboo
breeding area showed the greatest diversity and fairness, however, the plastic container had the
lowest indexes. Regarding the richness of the immatures found, it was showed greater richness
in the bamboo. The adults captured at sample point 5 of the Boa Esperança site had the highest
levels of diversity, equatability and richness. Evidence was found through the polymerase chain
reaction (RT-PCR), partial sequences of Zika vírus in sample of Hg. leucocelaenus, an
important species from the epidemiological point of view in the transmission of the yellow fever
vírus.

BANKING MEMBERS:
Externa à Instituição - RENATA CAMPOS AZEVEDO
Presidente - 387200 - FRANCISCO GERSON ARAUJO
Interno - 005.893.247-05 - JERONIMO AUGUSTO FONSECA ALENCAR - UFRJ
Externo à Instituição - RONALDO FIGUEIRÓ PORTELLA PEREIRA