Development and intrapuparial characterization of Peckia (Euboettcheria) collusor (Curran and Walley, 1934) (Diptera: Sarcophagidae), under different temperatures, with application in forensic entomology
Immature, Postmortem interval, flies
The Sarcophagidae family has approximately more than 3,000 species, recording great diversity in Brazil, flies strongly associated with medical, veterinary, sanitary, ecological and entomological studies due to their life habits. However, in forensic entomology, the sarcophagus family is still little used for studies, despite being strongly registered in carcasses and even in human cadavers. Therefore, this study aims to identify and compare the stages of development and intrapupariform morphological characteristics to serve as an auxiliary tool in forensic entomology. Pupae collected after the zero mark were killed at intervals of 3 in 3 hours until completing the first 24 hours and after 6 in 6 hours until the emergence of the first adults, for each time 30 pupae were used, totaling 1560 for 27ºC and 1290 for 32 ° C. The time of intrapupal development of Peckia (Euboettcheria)collusor under controlled laboratory conditions was 288 hours at 27 ° C and 228 hours at 32 ° C. The 2,850 pupae were identified according to the worked temperature, being classified into eight stages of possible: puparia; prepupae; anterior and posterior cryptocephalic pupa; phanerocephalic pupa; pupa-adult apolysis; adult farate and imago, in addition to obtaining 16 key morphological characters to identify the age of the pupae. The intrapupariform morphological characteristics obtained have great potential to estimate the minimum Postmortem Interval (minPMI) through the work of researchers, specialists, technical assistants or forensic entomologists.