Banca de DEFESA: TÂNIA CRISTINA DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : TÂNIA CRISTINA DA SILVA
DATE: 27/01/2022
TIME: 14:00
LOCAL: on-line
TITLE:

Penis morphology Sphiggurus villosus (F. Cuvier, 1823) (Erethizontidae, Rodentia)

KEY WORDS:

Histoarchitecture, copulatory organ, hedgehog

PAGES: 57
BIG AREA: Ciências Biológicas
AREA: Zoologia
SUBÁREA: Zoologia Aplicada
SUMMARY:

The Order Rodentia has a greater number of species, with significant diversity occupying different environments, habits and ecological niches. Studying comparative anatomy can provide information that will serve as a basis for large areas of biological sciences and biomedicine, and, based on this premise, the study aimed to describe the morphology of the S. Villosus hedgehog penis. Fifteen specimens donated by the Serra dos Órgãos National Park to the Department of Animal and Human Anatomy of the Federal Rural University of Rio de Janeiro (UFRRJ) were used. hese were fixed with perfusion of 10% formaldehyde solution, placed in polyethylene boxes and,later, dissected. The dissections were carried out with an incision in the preputial ostium and all the surrounding skin involving the region and the entire circumscribed area along with the perineal region, in order to expose the genitalia, as well as observe the anatomical regions of the penis, its morphology and the static muscles. From the anatomical characterization in loco, the penises were excised at the root and taken to the Integrative Histology Laboratory (UFRJ) to be included in the histological routine and histochemical and immunohistochemical techniques were performed.The macroscopic and microscopic findings of the penis of Sphiggurus villosus, allowed the identification of muscles. ischiocavernous and bulbospongiosus, constituting the root of the penis (radix penis). This cylindrical structure follows cranially arranged being continuous with the body (penis body). From each sample, cuts were made from the region of the penis body with 5 micrometers in thickness each, for each type of coloration. Hematoxylin-eosin, Mallory's Trichrome, orcein and Picrosirius red stains allowed the characterization of the elements of the extracellular matrix. The immunohistochemistry technique with anti-alpha-actin antibody was performed to mark the muscle cells. Scanning electron microscopy allowed the characterization of the surface of the penis of S. villosus, demonstrating the disposition and morphology of the spinous protuberances in the region of the glans penis. he results of the histochemical and immunohistochemical analysis of the penis of S. villosus revealed a musculocavernous type of histoarchitecture. he results of this research allow us to understand aspects of the reproductive behavior of these species, in order to improve the management of animals in captivity.

BANKING MEMBERS:
Interno - 387200 - FRANCISCO GERSON ARAUJO
Externa à Instituição - LYCIA DE BRITO GITIRANA - UFRJ
Interno - 2181722 - MARCELO ABIDU FIGUEIREDO
Externo à Instituição - PAULO DE SOUZA JUNIOR - Unipampa