Banca de QUALIFICAÇÃO: DIEGO MATTOS PENEDO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
DISCENTE : DIEGO MATTOS PENEDO
DATA : 30/05/2019
HORA: 09:00
LOCAL: Sala 30 do ICBS
TÍTULO:

Molecular, cytogenetic and population data analyzes of capuchin-monkeys, Sapajus nigritus (Primates: Cebidae), from Ilha da Marambaia, Mangaratiba, Rio de Janeiro

PALAVRAS-CHAVES:

Cytochrome Oxidase I and II, Cytochrome B, C-banding, fluorescence in situ hybridization, microsatellites, demography

PÁGINAS: 99
GRANDE ÁREA: Ciências Biológicas
ÁREA: Genética
SUBÁREA: Genética Animal
RESUMO:

Several studies have been carried out with robust and gracile capuchin-monkeys (Sapajus
and Cebus) in order to understand their evolutionary histories in Central and South
America. Genetic analyzes, in association with the characteristics of fur, aid in the
identification of interspecific relations and evidence the diversity presented by both genera.
In Brazil, the Sapajus species are predominantly distributed, being S. nigritus endemic to
the Atlantic Forest and characteristic of the southeast. The Ilha da Marambaia, an
important forest remnant in the Rio de Janeiro state, houses a population of capuchin-
monkeys that is still little studied, both in terms of genetic diversity and population data.
The aim of this study is to analyze this population through coat patterns, karyotype
evaluation with classical and molecular cytogenetic techniques and sequencing of three
mitochondrial genes, Cytochrome Oxidase I (MT-CO1), Cytochrome Oxidase II (MT-
CO2) and Cytochrome B (MT-CYB), comparing them with samples from four other
regions of Rio de Janeiro state, southeast of São Paulo and the literature. The genetic
diversity of the population will be further characterized, by the use of microsatellite

markers, and the demographics of groups in the region, through recordings with camera
traps. To date, 20 individuals from Ilha da Marambaia, five from Parque Nacional do
Itatiaia (PNI), at Itatiaia city, one from Parque Nacional da Serra dos Órgãos (PARNASO),
at Teresópolis city, one from Guaratiba, Rio de Janeiro city, eight from Reserva Biológica
(ReBio) de Poço das Antas, at Silva Jardim city and five from southeast São Paulo (SP)
were analyzed. The coat features of the analyzed individuals were consistent with that
described for S. nigritus, with coloration varying from brown to blackish on the back, with
limbs, tail and top of the head always dark, in addition to a yellow chest and a contrasting
lighter face. Five types of tufts of fur at the top of the head were observed, varying in size
and shape. Cytogenetic analyzes revealed karyotype with 2n=54,XX/XY for all
individuals. Extra-centromeric C-banding were observed in pairs 4, 12, 13 and 17 of all
individuals and in par 20 of the single PARNASO individual. Pair 11 presented a large and
terminal heterochromatic block, occupying approximately 80% of the long arm, for the
five individuals analyzed from the PNI and two from the southeastern of São Paulo. For
the other individuals the total deletion of constitutive heterochromatin was detected and
this chromosomic pair is a small acrocentric. Analysis of the MT-CO1 gene demonstrated
two well-delineated groups for S. nigritus, including the literature samples, being more
closely related to S. xanthosternos. For the MT-CO2 gene, there was a consistent grouping
of the samples from this study, although a sequence of S. xanthosternos from the literature
was included with little support. Samples of the literature for S. nigritus (from Argentina)
and S. cay grouped in a distinct branch. Regarding the MT-CYB gene, there was a
consistent grouping of the samples from this study with S. nigritus specimens from the
literature, although three specimens of S. xanthosternos and one of S. cay were included in
the same clade, although with little support. The C-band pattern of par 11 presented by
most of the analyzed individuals corresponds to that described for S. nigritus from
Argentina, as small and acrocentric, but the presence of the large and terminal
heterochromatic block for individuals from nearby regions of PNI and Southeast of SP
may indicate a biogeographical distribution pattern for polymorphisms. Through the MT-
CO1 and MT-CYB genes it was possible to perceive the grouping of the populations with
northern distribution (Ilha da Marambaia, Guaratiba, PARNASO, ReBio de Poço das
Antas and of literature - Angra dos Reis) and those with southern distribution (PNI,
southeast SP and of literature - from SP and Paraná state). Future analyzes of microsatellite
loci in association with demographic data will allow the evaluation of the genetic diversity
of the Illha da Marambaia capuchin-monkey population, as well as its viability.

MEMBROS DA BANCA:
Externo à Instituição - CARLOS EDUARDO DA SILVA VERONA - FIOCRUZ
Interno - 1539011 - DENISE MONNERAT NOGUEIRA
Interno - 2289640 - HELIO RICARDO DA SILVA
Externo à Instituição - LENA GEISE
Externo ao Programa - 3040326 - LEONARDO FERREIRA