Banca de DEFESA: ANDREOLI CORREIA ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANDREOLI CORREIA ALVES
DATE: 02/07/2021
TIME: 15:00
LOCAL: INSTITUTO DE ZOOTECNIA
TITLE:

Biotechnologies Applied to Fish Reproduction: Production of Mitotic Gynogenetics.

KEY WORDS:

Aquaculture, conservation, embryology, chromosome manipulation

PAGES: 71
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Produção Animal
SUMMARY:

ALVES, Andreoli Correia. Biotechnologies Applied to Fish Reproduction: Production of Mitotic Gynogenetics. 2021. 70 p. Dissertation (Master in Zootechnics). Instituto de Zootecnia, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ. 2021. Biotechnologies applied to reproduction are important tools to increase aquacultural production and support studies of conservation and maintenance of threatened species. Ginogenesis, is a uniparental reproductive process of exclusively maternal genetic inheritance, that may be induced in order to provide monosex population with increased performance, clonal lineages and may be used to elucidate the mechanisms of sex determination. Therefore, the objective of this study was to establish mitotic gynogenesis in Astyanax altiparanae, an important aquaculture and laboratory species. Four egg batches were used, each of them were divided into four treatments as follows: oocytes fertilized with normal semen (diploid control), oocytes fertilized with UV-inactivated semen without the application of thermal shock (haploid gynogenetic), oocytes fertilized with UV-inactivated semen succeeded by heat-shock (40°C, 24 minutes after fertilization -mpf) (diploid gynogenetic) and oocytes fertilized with normal semen with shock application 24 mpf (tetraploid). Fertilization and survival rates were checked in the main stages of early development (cleavage, blastula, gastrula, somite stage and hatching), as well as the percentage of normal and abnormal larvae. The newly hatched larvae (maximum of 20 samples repetition) from all treatments had ploidy status confirmed by flow cytometry and chromosome preparations. The results show that irradiation and thermal shock significantly reduced survival in the hatching stage (26.50 ± 2.50%, P > 0.05), normal larvae rate (36.62 ± 3.42%, P = 0.08) and provided increased rate of abnormal larvae (63.38 ± 3.42%, P = 0.07), and 98.64% of the total hatching confirmed mitotic gynogenetic larvae. tetraploidization gave a hatching rate of 17.8 ± 4.11% (P = 0.01) was observed with 10.64 ± 4.27% of normal larvae and 6.74 ± 2.52% of abnormal larvae, and 37.25% of the total number of hatched larvae confronting tetraploid.

BANKING MEMBERS:
Externa à Instituição - YARA AIKO TABATA - OUTRO
Externa ao Programa - 1051901 - MARINA MORTATI DIAS BARBERO
Presidente - 3067308 - MATHEUS PEREIRA DOS SANTOS