Banca de DEFESA: BRUNA CAROLINE FRANZAN

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : BRUNA CAROLINE FRANZAN
DATE: 26/02/2021
TIME: 13:00
LOCAL: INSTITUTO DE ZOOTECNIA
TITLE:

Diets, prebiotic and probiotic in the intestinal microbiome of equines.

KEY WORDS:

Complete diet, fermentation,fiber, Saccharomyces cerevisiae, simbiotic

PAGES: 183
BIG AREA: Ciências Agrárias
AREA: Zootecnia
SUBÁREA: Produção Animal
SUMMARY:

 

FRANZAN, Bruna Caroline. Diets, prebiotic and probiotic in the intestinal microbiome of equines. 2021. 161p. Thesis(Doctorate in Animal Science). Animal Science Institute. Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, 2021. The intestinal microbiota plays important role in horse’s nutrition and health.This work aimed to evaluate a complete extruded diet (CED) to fed horses and the dietary factors that influence the faecal microbiome. Horses fed a CED had lower water and fiber intakes, higher dry matter (DM), ether extract and gross energy intakes, and lower production and fecal pH. The DM, organic matter, ether extract, crude protein and crude energy digestibility were superior in the CED. The CED mean retention time (MRT) was shorter compared to the Coastcross hay diet (CHD), when obtained by LIPE^® marker.CHDintake for 21 days increased theabundance of Actinobacteria. The gradual adaptation of horses to the CED intake affected the phylum Verrucomicrobia, Synergistetes, Tenericutes and Lentisphaerae. The inclusion of 30% CED in the diet affects the abundance of bacterial groups in the fermentative activity, however, the diversity of bacterial species in the feces was maintained until the moment of 60% CED and 40% CHD intakes. The abrupt diet changefrom CHDto CED resulted in a reduction in faecal pH 96 hours after change and a bacterial diversityreduction 24 hours after chance. In addition, it affected the phyla Bacteroidetes, Firmicutes, Verrucomicrobia, Proteobacteria, Elusimicrobia and Actinobacteria. The abrupt changefrom CED toCHD resulted in a reduction in fecal pH 24 hours after the change and a bacterial species diversity increasesof 96 hours after change. In addition, it affected the phyla Synergistetes and Lentisphaerae. Finally, both diet changes resulted in an increase in the abundance of OTUs classified as Bacteroidetes and Treponema 24 hours after change. Supplementation with prebiotic inulin (PRE), probiotic Saccharomyces cerevisiae(PRO) and symbiotic inulin + S. cerevisiae(SIM) did not influence the bacterial diversity species observed in the feces of foals during weaning. The supplementation with PRE increased the Bacilli class in the feces of suckling foals. The fecal pH of foals supplemented with PRE was higher than the control group. PRO and SIM supplementation increased the classes Erysipelotrichia and Saccharimonadia, respectively, after three days, however, the effect was not prolonged. In addition, there was a difference in fecal microbiomes between the sexes of foals. Therefore, it is concluded that CED can be used to feed horses, as long as a gradual adaptation is made.The composition of the diet andadditives supplementation affect the fecal microbiome of horses and their response to dietary changes.

BANKING MEMBERS:
Externo à Instituição - HÉLIO CORDEIRO MANSO FILHO - UFRPE
Externo à Instituição - MÁRCIO CARVALHO COSTA
Externa ao Programa - 1815763 - IRENE DA SILVA COELHO
Externa ao Programa - 1350303 - MARIA IZABEL VIEIRA DE ALMEIDA
Presidente - 1899630 - VINICIUS PIMENTEL SILVA