ADDITION OF LITHOTHAMNIUM TO THE SOIL OF CONSTRUCTED WETLANDS TREATING CATTLE FARMING WASTEWATER: INFLUENCE ON NUTRIENT REMOVAL
constructed wetlands; Lithothamnium calcareum; Chrysopogon zizanioides; wastewater treatment; dairy cattle farming; nutrient removal.
Dairy cattle farming generates large volumes of wastewater with high organic and nutrient loads, representing an environmental challenge when discharged without adequate treatment. Vertical Flow Constructed Wetlands (VFCWs) are a sustainable alternative for treating these effluents but require optimization to maximize pollutant removal efficiency. This study evaluated the effect of different proportions of Lithothamnium calcareum (0%, 10%, 20%, and 30%) and the presence of vetiver grass (Chrysopogon zizanioides) on the removal efficiency of organic matter, nutrients, and physical parameters in VFCWs treating dairy wastewater. The experiment followed a randomized block design with a 4×2 factorial arrangement, totaling eight treatments with two replicates and eight sampling dates over two months (n=128 observations per parameter). The influent wastewater showed average concentrations of 3,383.1 mg/L COD, 89.4 mg/L ammoniacal nitrogen, 56.6 mg/L nitrate, 4.6 mg/L nitrite, 353.3 mg/L phosphate, 26,075 PtCo color, and 1,337.4 NTU turbidity. The results demonstrated that Lithothamnium calcareum had a statistically significant effect on the removal of COD (p = 0.004), color (p = 0.009), and turbidity (p = 0.003), with efficiencies ranging from 76.7% to 81.3% for COD, 74.9% to 96.6% for color, and 74.3% to 98.9% for turbidity. The LC 30% CV treatment showed the best performance for COD (81.3%) and turbidity (98.9%). The presence of Vetiver had a significant effect on ammoniacal nitrogen (p = 0.009) and nitrate (p = 0.013), with N-NH₄⁺ removal efficiencies ranging from 94.4% to 100.0%. Notably, the control treatment (LC 0% CV) showed higher efficiency for nitrate (84.4%), indicating negative interference of LC on denitrification processes. For nitrite and phosphate, no significant effects of the tested factors were detected, although the efficiencies were satisfactory (71.0-76.9% for NO₂⁻; 86.1-88.4% for PO₄³⁻). Chlorophyll assessment revealed a non-linear pattern with a minimum at LC 20%, suggesting possible competition for phosphorus between the plant and the substrate. It is concluded that Lithothamnium calcareum is a promising component for VFCWs aiming at the removal of organic matter and physical parameters, while Vetiver contributes to the transformation of nitrogen compounds, providing support for the optimization of agro-industrial wastewater treatment systems.