Banca de QUALIFICAÇÃO: JACOB SANTANA DE LIMA NETO

Uma banca de QUALIFICAÇÃO de DOUTORADO foi cadastrada pelo programa.
STUDENT : JACOB SANTANA DE LIMA NETO
DATE: 01/07/2026
TIME: 09:00
LOCAL: Sala 20 - Pós-Graduação
TITLE:

Potential for Producing Bioproducts from Microalgal Biomass Cultivated in Agro-industrial Wastewater

KEY WORDS:

lipids; bioremediation; fatty acids; agro-industrial wastewater; bioenergy.

PAGES: 40
BIG AREA: Ciências Agrárias
AREA: Engenharia Agrícola
SUBÁREA: Engenharia de Água e Solo
SPECIALTY: Conservação de Solo e Água
SUMMARY:

Microalgae are emerging as a promising alternative for the removal of nutrients and contaminants from wastewater. Bioremediation is a key advantage of microalgae; furthermore, the resulting biomass can serve as feedstock for energy conversion—specifically the production of biodiesel and bioethanol—as well as for applications in biofertilizers, cosmetics, pigments, pharmaceuticals, and dietary supplements. The overall objective of this thesis is to investigate the potential of microalgae cultivated in agro-industrial wastewater for the production of bioproducts, focusing on biodiesel and biofertilizers. The research will be conducted in three stages. The first stage, which will form Chapter 1, proposes the use of synthetic soy milk wastewater (SSMW) subjected to varying ozonation times; the goal is to increase the availability of nitrogenous compounds, thereby promoting microalgal cultivation and biomass production, enhancing lipid accumulation, and consequently increasing biodiesel yield. The methodology involves treating the SSMW with ozone for durations of 0, 10, 20, 30, 60, 90, 120, 150, and 180 minutes. Subsequently, the SSMW sample showing the best performance regarding nitrogenous compound accumulation will be used as the culture medium for microalgae in column photobioreactors with a working volume of 3 L. Analyses will be conducted on biomass growth, nutrient removal kinetics, CO2 biofixation quantification, and biomass biochemical characterization. The second and third stages, which will comprise Chapter 2, aim to cultivate *Chlorella* sp. microalgae. ...in dairy wastewater (DWW) under different light spectra, aiming to maximize biomass production, bioremediate the DWW, contribute to CO₂ biofixation, and enable the production and application of microalgae-based biofertilizer for cowpea (*Vigna unguiculata* – cv. BRS Novaera) cultivation. The methodology involves cultivation in photobioreactors; the use of blue, green, red, yellow, and white LED spectra; assessment of microalgal biomass growth; physicochemical analyses of the DWW, biofertilizer, and soil; and agronomic trials. Cultivation will consist of three treatments with 25 replicates each—totaling 75 experimental units—conducted in pots. The treatments are: T1 – water (control), T2 – urea (conventional fertilizer), and T3 – microalgae-based biofertilizer. The pot specifications for the BRS Novaera cultivar will be: capacity – 10 L; width (top) – 24.5 cm; width (bottom) – 19 cm; height – 29 cm. Results will be analyzed using Analysis of Variance (ANOVA), a statistical method widely used to test the hypothesis of equality among the means of three or more experimental groups. Prior to ANOVA, tests will be conducted to verify assumptions regarding homogeneity of variance, normality, and independence of residuals, ensuring the validity and robustness of the results. Once these assumptions are confirmed, Tukey's test will be applied at a 5% significance level to perform multiple comparisons among treatment means and identify those showing statistically significant differences. All statistical analyses will be performed using R software, version 4.2.3.

COMMITTEE MEMBERS:
Presidente - 1133133 - HENRIQUE VIEIRA DE MENDONCA
Interno - 2131822 - CONAN AYADE SALVADOR
Externa ao Programa - 1400897 - ALICIA ZEM FRAGA - UFRRJ