Banca de DEFESA: LÍVIA DA ROCHA NATALINO MONTEIRO

Uma banca de DEFESA de DOUTORADO foi cadastrada pelo programa.
STUDENT : LÍVIA DA ROCHA NATALINO MONTEIRO
DATE: 24/02/2021
TIME: 09:00
LOCAL: Web Conferência
TITLE:

ANALYSIS OF THE DORSAL RAFE NUCLEUS TRANSCRIPTOME AFTER CHALLENGES TO HYDROELECTROLYTIC AND ENERGY HOMEOSTASIS

KEY WORDS:

Transcriptome, Sodium appetite, Food deprivation

PAGES: 99
BIG AREA: Ciências Biológicas
AREA: Fisiologia
SUBÁREA: Fisiologia de Órgãos e Sistemas
SPECIALTY: Neurofisiologia
SUMMARY:

The sodium ion is as a key element in the regulation of the hydroelectrolytic balance, so that its levels must be kept in a narrow range compatible with life. For such regulation, a series of renal, endocrine, and behavioural mechanisms are activated. The dorsal raphe nucleus (DRN) has been implicated as an important brain nucleus involved in the modulation of food-intake behaviour, especially sodium-specific appetite. In this work we investigate the transcriptomic changes in the DRN after challenges to hydroelectrolytic and energy homeostasis. Male Wistar rats, approximately 60 days old, were randomly separated into five experimental groups: a) control (CTRL); b) low sodium diet (LS); c) furosemide (FURO); d) salt loading (SL); and (e) food deprivation for 48 hours. After the experimental period, euthanasia was performed to collect blood and brain. Animals submitted to treatment with furosemide and saline overload showed a significant increase in hematocrit (FURO 12.4%, SL 14,9%) and reduction (FURO 1.25%) and increase in plasma sodium (SL 13%), respectively. Animals subjected to food deprivation showed an increase in hematocrit (13%) and a reduction in body weight (12.9%). The DRN RNA sequencing resulted in approximately 18,600 genes mapped in total. Among the most expressed genes, tryptophan hydroxylase isoform 2 (Tph2), the gene encoding the serotonin transporter SERT (Slc6a4) and the vesicular monoamine transporter (Slc18a2) were found, confirming the prevalence of serotonergic neurons. The comparison of differentially expressed genes using the Venn diagram revealed a low altered number in the FURO group (6 genes), no altered genes in the DP group and 22 genes altered by saline overload. Based on the results, we performed the RT-qPCR validation only for the saline overload group. Among the 22 altered genes in the transcriptome, we selected 10 targets for RT-qPCR, among which, 7 genes were successfully validated. Finally, the sequencing of the group subjected to food deprivation revealed 108 altered genes, when comparing this result to that obtained in salt loading, we saw that the groups have 6 altered genes in common (C3, Etnppl, Gjb6, RT1-T24-4, Slc35d3 e Sult1a1). Despite the need to validate the results of food deprivation, they indicate a possible overlap in the level of DRN between mechanisms that regulate specific sodium appetite and eating behaviour.

BANKING MEMBERS:
Presidente - 103.378.127-48 - ANDRE DE SOUZA MECAWI - UNIFESP
Interno - 386960 - WELLINGTON DA SILVA CORTES
Externo à Instituição - LAURIVAL ANTONIO DE LUCA JUNIOR - UNESP
Externa à Instituição - SILVIA GRACIELA RUGINSK LEITÃO - UNIFAL-MG
Externo à Instituição - HÉLIO ZANGROSSI JÚNIOR - USP