Banca de DEFESA: NATALIA D' ASSUMPÇÃO LIMA RANGEL

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : NATALIA D' ASSUMPÇÃO LIMA RANGEL
DATA : 08/03/2018
HORA: 09:00
LOCAL: Sala 01 Pavilhão de Química
TÍTULO:

Maternal high fat diet consumption: change in the angiotensina II receptor expression (AT1) and left ventricular hypertrophy in the offspring at weaning.

PALAVRAS-CHAVES:

high fat diet, metabolic programming, cardiac hypertrophy

PÁGINAS: 98
GRANDE ÁREA: Ciências Biológicas
ÁREA: Fisiologia
SUBÁREA: Fisiologia Geral
RESUMO:

Obesity has become a public health problem in the world. Overweight pregnant women can cause metabolic programming in the lineage, with the emergence of type 2 diabetes, dyslipidemias and cardiovascular diseases in the adult progeny. Our research group has previously shown that maternal consumption of a high fat diet promoted increased body mass, adiposity, hyperleptinemia in the offspring of rats at weaning, as well as impairment of systolic function at 30 days of age. The renin angiotensin system (RAS) expressed in the heart appears to trigger cardiac hypertrophy (CH), through the overexpression of Angiotensin II (Ang II) and activation of it’s receptor, AT1. This study aimed to investigate whether the maternal consumption of a high fat diet promotes CH, alter the gene expression of components involved in the contraction of the heart and correlate such changes to the cardiac RAS system in male and female offspring. For this, Wistar rats received control diet (9% lipids, group C) or high fat (29% lipids, HF group) for 8 weeks before mating, and during gestation and lactation. At weaning, 21 days of life, the offspring were weighed and euthanized. The heart and adipose white tissue (retroperitoneal, inguinal and perigonadal) were weighed. Histological analyzes (sections stained with HE and picrosirius) were realized using samples from the ventricles and biochemical and molecular analyzes (RIA and qPCR) were performed using left ventricular (LV) samples and AT1 receptor expression was assessed by Western blotting (Protocol: 017/2014-CEUA/IB/UFRRJ). Females and males of the HF progeny presented higher body mass, adiposity, LV hypertrophy, but did not present fibrosis; higher mRNA expression of Nppn; and less expression of β-MHC, SERCA2a and Ryr2. mRNA expression of α-MHC was reduced only in males of the DH offspring. There was no difference in Ang II dosage among the offspring. Protein expression of AT1 was higher in females of the HF progeny, but was not altered in males. These results suggest that the maternal consumption of high fat diet promotes LV hypertrophy in females and males of the HF offspring, however we verified alteration in the AT1 protein expression only in females of HF progeny, which suggests that the RAS system may be involved with HC observed in the females and not in the male animals of the HF offspring.

MEMBROS DA BANCA:
Interno - 2623617 - ANDERSON LUIZ BEZERRA DA SILVEIRA
Externo à Instituição - LUCIANE CLÁUDIA BARCELLOS DOS SANTOS SOUZA - UFRJ
Presidente - 2479104 - NORMA APARECIDA DOS SANTOS ALMEIDA