Banca de DEFESA: JOSÉ EDGARD DE OLIVEIRA ALVES

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JOSÉ EDGARD DE OLIVEIRA ALVES
DATA : 23/08/2019
HORA: 13:00
LOCAL: Sala 01 - PQ
TÍTULO:

Evaluation of hydroeletrolitic balance in normotensive Wistar rats and spontantaneous hypertensive rats

 

PALAVRAS-CHAVES:

Body fluids, sodium, blood pressure.

PÁGINAS: 90
GRANDE ÁREA: Ciências Biológicas
ÁREA: Fisiologia
SUBÁREA: Fisiologia Geral
RESUMO:

Given the close relationship between body fluid volume, sodium load and blood pressure, the present study aimed to demonstrate that SHR rats show differences in hydromineral balance compared to normotensive rats and are reflected in lower efficiency. in controlling the balance of water and osmols in the body. Male Wistar and SHR rats, aged between 90 and 120 days old, were submitted to four experimental protocols: (1) blood pressure evaluation and SHR model validation; (2) basal assessment and 24-hour water deprivation in metabolic cages; (3) plasma evaluations; and (4) assessment of 24-hour deprivation and water resubmission in metabolic cage. Our results showed significant differences in several studied parameters, among which we can highlight discrepancies in daily basal water intake (Wistar 15.35 ± 0.522 mL/100g vs. SHR 10.90 ± 0.228 mL/100g; p<0.0001); basal urinary excretion (Wistar 6.153 ± 0.227 mL/100g vs. SHR 4.773 ± 0.122 mL/100g; p<0.0001); urinary sodium load (SHR 0.6547 ± 0.019 mEq/100g/24h vs. 0.4866 ± 0.017 mEq/100g/24h Wistar; p<0.0001) and the urinary potassium load (SHR 1.381 ± 0.085 mEq/100g/24h vs. Wistar 1.146 ± 0.038 mEq/100g/24h; p=0.0137). The AVP and ANP levels were influenced by the water deprivation factor (F_(1,56) =13,20, p=0,0006; and F_(1,37) = 10,54; p=0,0025, respectively). whereas ANG II is influenced by water deprivation (F_(1,53) = 8,173; p=0,0061), without influence of strain factor (F_(1,53) = 0,3370; p=0,5640), but with interaction between them (F_(1,53) = 4,367; p=0,0415) and OT showed significance for the deprivation factor (F_(1,54) = 20,75; p<0,0001) and strain factor (F_(1.54) = 14.20; p=0.0004), and interaction between them occurred (F_(1,54) = 9.826; p=0.0028). In addition, during water resubmission the SHR rats presented lower water intake, whose two-way analysis of variance showed influence of the time factor (F_{(7, 245)} = 98.03; p<0.0001), of the strain factor (F_{(1, 35)} = 31.90; p<0.0001) and interaction (F_{(7, 245)} = 10.19; p<0.0001); and higher urinary volume, with influence of time and strain factors (F_{(7, 245)} = 10.19, p<0.0001; F_{(1, 70)} = 26.69, p<0.0001; respectively), although no interaction occurs. Thus, these results show discrepancies in the hydromineral balance between normotensive rats and SHR and reiterate the importance of integrating osmoregulatory and cardiovascular systems for body fluid homeostasis.

MEMBROS DA BANCA:
Interno - 103.378.127-48 - ANDRE DE SOUZA MECAWI - UNIFESP
Externo à Instituição - DANIEL BADAUE PASSOS JUNIOR - UFS
Interno - 2225596 - EMERSON LOPES OLIVARES